Stock No: 011108
Protocol 19185: Separated PCR Assay - Generic mCherry
Version 3.0

Notes

This assay will NOT distinguish hemizygous from homozygous transgenic animals.

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Transgene = 288 bp

Internal positive control = 324 bp

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note
9703 AGG ACG GCG AGT TCA TCT AC Transgene Forward mCherry
9704 TGG TGT AGT CCT CGT TGT GG Transgene Reverse mCherry
oIMR7338 CTA GGC CAC AGA ATT GAA AGA TCT Internal Positive Control Forward
oIMR7339 GTA GGT GGA AAT TCT AGC ATC ATC C Internal Positive Control Reverse

Strains Using This Protocol

Stock Number Strain Name
024046 C57BL/6-Tg(Csf1r-HBEGF/mCherry)1Mnz/J
018542 FVB-Tg(Kdr-mCherry)1Medi/J
018067 FVB-Tg(Prism)1849Htz/J
018071 FVB-Tg(Prism)1861Htz/J
014177 STOCK Tg(Afp-mCherry)1Hadj/J
025693 STOCK Tg(CAG-GFP,-mCherry/Pxn)#Mgin/KleyJ
017470 STOCK Tg(Tnc-mCherry)2Pmay/J
011108 STOCK Tg(Ttr-RFP)1Hadj/J
8 strains use this protocol