This assay does not work well without the use of a Hotstart Taq polymerase.
(a) We want to keep this as a generic statement, so that it covers the multiple
Hotstart Taqs we currently use, and if we change vendors in the future.
This assay will not distinquish hemizygous from homozygous transgenic animals.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.