Stock No: 010720
Protocol 22130: QPCR Assay - Fgf2<tm3Doe>
Version 3.0


The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note
11664 Fluorophore-1 CCC AAG AGC TGC CAC AG Quencher-1 Transgene Forward A
11665 Fluorophore-2 GGG AAG CGA GGT GAT GC Quencher-2 Transgene Reverse A
13798 Fluorophore-3 AGA CTA GGG GGC CGC GGA AG Quencher-3 Tg Probe
oIMR1544 CAC GTG GGC TCC AGC ATT Internal Positive Control Forward A
oIMR3580 TCA CCA GTC ATT TCT GCC TTT G Internal Positive Control Reverse A
TmoIMR0105 Fluorophore-4 CCA ATG GTC GGG CAC TGC TCA A Quencher-4 IC Probe

Reaction A

Component Final Concentration
Kapa Probe Fast QPCR 1.00 X
11664 0.40 uM
11665 0.40 uM
oIMR1544 0.40 uM
oIMR3580 0.40 uM
Tg Probe 0.15 uM
IC Probe 0.15 uM


Step Temp °C Time Note
1 95.0 --
2 95.0 --
3 60.0 -- repeat steps 2-3 for 40 cycles
JAX uses a very high speed Taq (~1000 bp/sec), use cycling times recommended for your reagents.

Strains Using This Protocol

This is the only strain that uses this protocol.