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Mutant =C/C
Heterozygote = T/C
Wild type = T/T
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
21329 | ACA GCC TGA ATC CAA CTT AGG | Forward | ||||
21330 | AAA TGT AAC ACG GTT GCT AGA ATG | Reverse | ||||
21331 | Fluorophore-1 | AGA TGG GCT GGT AAG TGA TAA CT | Quencher-1 | |||
21332 | Fluorophore-2 | GAT GGG CTG GCA AGT GAT AAC | Quencher-2 |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 0.42 |
ddH2O | |
Primer 1 | 0.03 |
Primer 2 | 0.03 |
Primer 3 | 0.02 |
Primer 4 | 0.02 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |