Stock No: 008832
Protocol 11187: End Point Analysis Assay - Tshr<hyt-2J>
Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Mutant = T/T
Heterozygote = C/T
Wild type = C/C

 

 

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note
18413 TCA CTG TTT TCG CCA GTG AG Forward
18414 GAT GCT GAC CTT GGC ATA GC Reverse
18415 Fluorophore-1 ACC CTG GAG CGA TGG TAC G Quencher-1
18416 Fluorophore-2 CAC CCT GGA GTG ATG GTA CGC Quencher-2

Reaction A

Component Final Concentration
Kapa Probe Fast QPCR 0.95
Primer 1 0.22
Primer 2 0.22
0.00
0.00
Primer 3 0.63
0.00
DNA

Cycling

Step Temp °C Time Note
1 95.0 --
2 92.0 --
3 60.0 --
4 -- repeat steps 2-3 for 40 cycles
5 4.0 -- Forever
JAX uses a very high speed Taq (~1000 bp/sec), use cycling times recommended for your reagents.

Strains Using This Protocol

This is the only strain that uses this protocol.