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Mutant = T/T
Heterozygote = C/T
Wild type = C/C
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
18413 | TCA CTG TTT TCG CCA GTG AG | Forward | ||||
18414 | GAT GCT GAC CTT GGC ATA GC | Reverse | ||||
18415 | Fluorophore-1 | ACC CTG GAG CGA TGG TAC G | Quencher-1 | |||
18416 | Fluorophore-2 | CAC CCT GGA GTG ATG GTA CGC | Quencher-2 |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 0.95 |
Primer 1 | 0.22 |
Primer 2 | 0.22 |
0.00 | |
0.00 | |
Primer 3 | 0.63 |
0.00 | |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 92.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 4.0 | -- | Forever |