Protocol 21404: Standard PCR Assay - Tg(FXN) repeat copy number
Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Number of Repeats depends on JR and mouse.
Flanking Sequence: 498 bp (subtract from PCR 
size and divide by 3 for # of repeats present for each animal)

Plasmid Control Expected Size: 3094bp

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note
43604 GGC TTG AAC TTC CCA CAC GTG TT
43605 AGG ACC ATC ATG GCC ACA CTT

Strains Using This Protocol

Stock Number Strain Name
008586 B6.Cg-Tg(FXN*)1Sars Fxntm1Mkn/J
030395 STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/800J
030930 STOCK Fxnem2.1Lutzy Tg(FXN)YG8Pook/J
024097 STOCK Fxntm1Mkn Tg(FXN)Y47Pook/J
024113 STOCK Fxntm1Mkn Tg(FXN)YG8Pook/2J
5 strains use this protocol