Stock No: 008462
Protocol 21006: Separated PCR Assay - GAL Panel-B6J vs. 129S
Version 1.0


The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer 5' Label Sequence 5' → 3' 3' Label Primer Type Reaction Note

Strains Using This Protocol

Stock Number Strain Name
008462 B6.129P2-Trp53tm1Brn/J
006440 B6.129S4-Ptentm1Hwu/J
017009 B6.129X1-Nfe2l2tm1Ywk/J
006381 B6;129S-Stxbp1tm1Sud/J
027992 B6J.129S7(Cg)-Mir22tm1.1Arod/J
028867 B6J.Cg-Gad2tm2(cre)Zjh/MwarJ
010802 STOCK Gad2tm2(cre)Zjh/J
7 strains use this protocol