Protocol 20644: Standard PCR Assay - Slc6a4<tm1Kpl> Alternate1

B6.129(Cg)-Slc6a4^tm1Kpl/J

Stock No: 008355

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

Mutant = ~190 bp
Heterozygote = ~190 bp and 237 bp
Wild type = 237 bp

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note
30801		CCT GTC TGT CAT TGG CTA TGC		Wild type Forward
30802		GCT GAC TGG AGA TCA GGC TA		Common
oIMR6773		TGG CTA CCC GTG ATA TTG CT		Mutant Forward

Strains Using This Protocol

This is the only strain that uses this protocol.