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Publications are available which describe other approaches to genotyping this strain. One of these can be found at: https://www.nature.com/articles/aps201752
Mut=T
Wt=G
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
14349 | ACC AAC TTC CCA ACA GTC CA | Forward | A | |||
14350 | TGA TGC CCT GAA AAT CAA GC | Reverse | A | |||
17934 | Fluorophore-1 | TTT GAT GGA GGG AAA CAA ACC TA | Quencher-1 | WT Probe | ||
17935 | Fluorophore-2 | TTT TGA TGG AGG TAA ACA AAC CTA A | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
14349 | 0.40 uM |
14350 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
002048 | B6 x C57BLKS-Dock7m Leprdb Myo15sh2-J/J |
000697 | B6.BKS(D)-Leprdb/J |
000699 | B6.Cg-Dock7m Leprdb/+ +/J |
000642 | BKS.Cg-Dock7m +/+ Leprdb/J |
000700 | BKS.Cg-Dock7m Leprdb/+ +/J |
031579 | BKS.Cg-Dock7m+/+ Leprdb Tg(SLC2A4)HJdm/PfzrJ |
008340 | BKS.Cg-Leprdb Nos3tm1Unc/RhrsJ |
001192 | BKS.Cg-meaJ Leprdb +/+ + Dock7m/J |
000707 | CBA.Cg-Dock7m Leprdb/+ +/J |
012666 | D2.BKS(D)-Leprdb/J |
006654 | FVB.BKS(D)-Leprdb/ChuaJ |
11 strains use this protocol |