Stock No: 008320
Protocol 20626: Probe Assay - Generic Cre Probe
Version 3.0
Notes
This assay will NOT distinguish hemizygous from homozygous transgenic animals.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Expected Results
Transgene Tm = 84°C +/- 1.0°C
Internal positive control Tm = 79.4°C+/- 1.0°C
Transgene = 102 bp
Internal Positive Control = 74 bp
JAX Protocol
Protocol Primers
| Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
|---|---|---|---|---|---|---|
| 13593 | Fluorophore-1 | AAA CAT GCT TCA TCG TCG GTC CGG | Quencher-1 | Tg Probe | Cre | |
| oIMR1084 | GCG GTC TGG CAG TAA AAA CTA TC | Transgene Forward | A | Cre | ||
| oIMR1085 | GTG AAA CAG CAT TGC TGT CAC TT | Transgene Reverse | A | Cre | ||
| oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
| oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
| TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Reaction A
| Component | Final Concentration |
|---|---|
| Kapa Probe Fast QPCR | 1.00 X |
| ddH2O | |
| oIMR1084 | 0.40 uM |
| oIMR1085 | 0.40 uM |
| oIMR1544 | 0.40 uM |
| oIMR3580 | 0.40 uM |
| Wt Probe | 0.15 uM |
| Mutant Probe | 0.15 uM |
| DNA |
Cycling
| Step | Temp °C | Time | Note |
|---|---|---|---|
| 1 | 95.0 | -- | |
| 2 | 95.0 | -- | |
| 3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
JAX uses a very high speed Taq (~1000 bp/sec), use cycling times recommended for your reagents.
Strains Using This Protocol
| Stock Number | Strain Name |
|---|---|
| 005989 | 129;FVB-Tg(PTH-cre)4167Slib/J |
| 017789 | 129S-Slc11a2tm2Nca/J |
| 007179 | 129S.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/J |
| 007915 | 129S.FVB-Tg(Amh-cre)8815Reb/J |
| 008523 | 129S6.Cg-Tg(NPHS2-cre)295Lbh/BroJ |
| 030182 | B6(129S4)-Foxc2tm1.1(cre/ERT2)Rssr/J |
| 033169 | B6(129S4)-Kiss1tm1.1(cre/EGFP)Rpa/J |
| 032051 | B6(129S4)-Kittm1.1(cre)Jmol/J |
| 009575 | B6(129S4)-Et(cre/ERT2)119Rdav/J |
| 009580 | B6(129S4)-Et(cre/ERT2)1382Rdav/J |
| 009581 | B6(129S4)-Et(cre/ERT2)1642Rdav/J |
| 009582 | B6(129S4)-Et(cre/ERT2)1645Rdav/J |
| 009583 | B6(129S4)-Et(cre/ERT2)1957Rdav/J |
| 009584 | B6(129S4)-Et(cre/ERT2)2007Rdav/J |
| 009585 | B6(129S4)-Et(cre/ERT2)2047Rdav/J |
| 009574 | B6(129S4)-Et(cre/ERT2)21Rdav/J |
| 009577 | B6(129S4)-Et(cre/ERT2)296Rdav/J |
| 009578 | B6(129S4)-Et(cre/ERT2)398Rdav/J |
| 009573 | B6(129S4)-Et(cre/ERT2)4Rdav/J |
| 009579 | B6(129S4)-Et(cre/ERT2)837Rdav/J |
| 029290 | B6(Cg)-Sgtatm1.1Drmcr/J |
| 021072 | B6(Cg)-Slc6a8tm1.2Clar/J |
| 033895 | B6(Cg)-Tg(Snap25-FLPe)47DSJDJdd Tg(Egr1-cre/ERT2)47DSJDJdd/J |
| 021012 | B6(D2)-Tg(CAG-Brainbow1.0)3Eggn/J |
| 021881 | B6.129(Cg)-Arctm1.1(cre/ERT2)Luo/J |
| 018057 | B6.129(Cg)-Cul7tm2.1Jdec/J |
| 008320 | B6.129(Cg)-Leprtm2(cre)Rck/J |
| 033964 | B6.129(Cg)-Ptf1atm2(cre/ESR1)Cvw Krastm4Tyj Ptentm1Hwu/J |
| 029228 | B6.129-Bhlha15tm3(cre/ERT2)Skz/J |
| 018445 | B6.129-Mre11atm1.1Rchd/J |
| 025567 | B6.129-Olig2tm1.1(cre)Wdr/J |
| 005697 | B6.129-Otx1tm4(cre)Asim/J |
| 021160 | B6.129P2(Cg)-Cx3cr1tm2.1(cre/ERT2)Litt/WganJ |
| 018054 | B6.129P2(Cg)-Pitx2tm1.1Dmm/J |
| 023726 | B6.129P2-C1qatm1.1Tenn/J |
| 034597 | B6.129P2-Kif13aGt(AF0553)Wtsi/AhcJ |
| 016934 | B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J |
| 018997 | B6.129S(FVB)-Lhx3tm1.1Sjr/J |
| 032621 | B6.129S-Ly6atm1.1(cre)Jmol/J |
| 006600 | B6.129S1-Mnx1tm4(cre)Tmj/J |
| 016132 | B6.129S4-Perptm2Att/J |
| 006447 | B6.129S6(CBA)-Cebpatm1Dgt/J |
| 012900 | B6.129S6-Stat5btm1Mam Stat5atm2Mam/Mmjax |
| 025929 | B6.129S7(Cg)-Pik3catm1Jdo/J |
| 006054 | B6.C-Tg(CMV-cre)1Cgn/J |
| 030644 | B6.C-Tg(S100a4-cre)1Egn/JhrsJ |
| 008601 | B6.Cg-7630403G23RikTg(Th-cre)1Tmd/J |
| 006230 | B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J |
| 024521 | B6.Cg-Eif4g1tm1.2Mjff/J |
| 032923 | B6.Cg-Kittm1(cre)Htng/J |
| 030544 | B6.Cg-Npy1rtm1.1(cre/GFP)Rpa/J |
| 030546 | B6.Cg-Satb2tm1.1(cre/GFP)Rpa/J |
| 017889 | B6.Cg-Shank3tm1.1Bux/J |
| 032488 | B6.Cg-Six2tm2(cre/ERT2)Amc/J |
| 022865 | B6.Cg-Trib2tm1.1(cre/ERT2)Hze/J |
| 028538 | B6.Cg-Zbtb46tm3.1(cre)Mnz/J |
| 017346 | B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J |
| 006149 | B6.Cg-Tg(ACTA1-cre)79Jme/J |
| 022357 | B6.Cg-Tg(Arc-cre/ERT2)MRhn/CdnyJ |
| 027310 | B6.Cg-Tg(Camk2a-cre)2Szi/J |
| 027400 | B6.Cg-Tg(Camk2a-cre)3Szi/J |
| 033345 | B6.Cg-Tg(Cdh5-COP4*L132C,-lacZ)B28-2Mik/J |
| 032889 | B6.Cg-Tg(Cdh5-RHO/Adrb2,-lacZ)B27-3Mik/J |
| 009352 | B6.Cg-Tg(CDX2-cre*)189Erf/J |
| 016241 | B6.Cg-Tg(Col1a1-cre/ERT2)1Crm/J |
| 016237 | B6.Cg-Tg(Col1a2-cre/ERT,-ALPP)7Cpd/J |
| 006368 | B6.Cg-Tg(Cr2-cre)3Cgn/J |
| 006663 | B6.Cg-Tg(Eno2-cre)39Jme/J |
| 035668 | B6.Cg-Tg(Gata1-cre)1Sho/MdfJ |
| 009642 | B6.Cg-Tg(GH1-cre)1Sac/J |
| 003573 | B6.Cg-Tg(Ins2-cre)25Mgn/J |
| 003967 | B6.Cg-Tg(Rbp3-cre)528Jxm/J |
| 008863 | B6.Cg-Tg(Tek-cre)1Ywa/J |
| 012328 | B6.Cg-Tg(Tyr-cre/ERT2)13Bos/J |
| 028353 | B6.Cg-Tg(Vil1-EGFP)106Gum/J |
| 024688 | B6.FVB(129S)-Tg(Pax6-GFP/cre)1Rilm/J |
| 006451 | B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J |
| 028736 | B6.FVB(Cg)-Tg(Mylpf-cre)3Kraj/Mmjax |
| 028020 | B6.FVB-Tg(Adipoq-cre)1Evdr/J |
| 006137 | B6.FVB-Tg(Cdh5-cre)7Mlia/J |
| 018980 | B6.FVB-Tg(Ddx4-cre)1Dcas/KnwJ |
| 003724 | B6.FVB-Tg(EIIa-cre)C5379Lmgd/J |
| 011069 | B6.FVB-Tg(Gh1-cre)bKnmn/J |
| 011038 | B6.FVB-Tg(Myh6-cre)2182Mds/J |
| 003394 | B6.FVB-Tg(Zp3-cre)3Mrt/J |
| 021961 | B6;129-Abcg2tm3.1(cre/ERT2)Bsor/J |
| 017601 | B6;129-Eif2s1tm1Rjk/J |
| 024767 | B6;129-Fostm1Mxu/Mmjax |
| 016210 | B6;129-Lrrk2tm3.1Shn/J |
| 018319 | B6;129-Plxnd1tm1.1Tmj/J |
| 009600 | B6;129-Six2tm3(EGFP/cre/ERT2)Amc/J |
| 018773 | B6;129-Sult2b1tm1Rus/J |
| 024860 | B6;129-Tg(Drd1-cre)120Mxu/Mmjax |
| 022864 | B6;129S-Rasgrf2tm1(cre/folA)Hze/J |
| 017593 | B6;129S-Sox2tm1(cre/ERT2)Hoch/J |
| 009388 | B6;129S1-Osr2tm2(cre)Jian/J |
| 002914 | B6;129S4-Hexbtm1Rlp/J |
| 018019 | B6;129S4-Srsf2tm1Xdfu/J |
| 009576 | B6;129S4-Et(cre/ERT2)278Rdav/J |
| 017495 | B6;129S7-Crim1tm1(GFP/cre/ERT2)Pzg/J |
| 018937 | B6;C-Tg(Erv4-cre/ERT2*)657Slp/J |
| 009616 | B6;C3-Tg(A930038C07Rik-cre)4Aibs/J |
| 008844 | B6;C3-Tg(Ctgf-cre)2Aibs/J |
| 008839 | B6;C3-Tg(Cyp39a1-cre)1Aibs/J |
| 009117 | B6;C3-Tg(Cyp39a1-cre)7Aibs/J |
| 009111 | B6;C3-Tg(Scnn1a-cre)1Aibs/J |
| 009112 | B6;C3-Tg(Scnn1a-cre)2Aibs/J |
| 009103 | B6;C3-Tg(Wfs1-cre/ERT2)3Aibs/J |
| 025820 | B6;CBA-Tg(CRH-MAPT/Topaz)1Rck/J |
| 031779 | B6;CBA-Tg(Cyp19a1-cre)1Dlc/J |
| 024926 | B6;D2-Tg(Fshr-cre)1Ldu/J |
| 003466 | B6;D2-Tg(Sycp1-cre)4Min/J |
| 018402 | B6;FVB-Nrn1tm1.2Ndiv/J |
| 023748 | B6;FVB-Tg(Aldh1l1-cre)JD1884Htz/J |
| 003734 | B6;FVB-Tg(GZMB-cre)1Jcb/J |
| 026526 | B6;SJL-Akap6tm1.1Mskf/Mmjax |
| 004426 | B6;SJL-Tg(Cga-cre)3Sac/J |
| 003554 | B6;SJL-Tg(Col2a1-cre)1Bhr/J |
| 017738 | B6;SJL-Tg(Foxl1-cre)1Khk/J |
| 005249 | B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J |
| 003552 | B6129-Tg(Wap-cre)11738Mam/J |
| 007252 | B6Ei.129S4-Tg(Prm-cre)58Og/EiJ |
| 034731 | B6N(129S4)-Srd5a3tm1c(EUCOMM)Wtsi/J |
| 017704 | B6N.129-Pik3catm1Jjz/J |
| 017911 | B6N.129S6(Cg)-Esr1tm1.1(cre)And/J |
| 016881 | B6N.Cg-Adgra2tm1.1Bstc/J |
| 019102 | B6N.Cg-Tg(CAG-cre/Esr1*)5Amc/CjDswJ |
| 018966 | B6N.Cg-Tg(Camk2a-cre)T29-1Stl/J |
| 018965 | B6N.Cg-Tg(Fabp4-cre)1Rev/J |
| 018960 | B6N.Cg-Tg(Ins2-cre)25Mgn/J |
| 018964 | B6N.Cg-Tg(KRT14-cre)1Amc/J |
| 019103 | B6N.Cg-Tg(Nes-cre)1Kln/CjDswJ |
| 019099 | B6N.FVB-Tmem163Tg(ACTB-cre)2Mrt/CjDswJ |
| 030894 | B6NCrl.129P2(B6)-Apoetm1Unc Ccr5tm1Blck/BlckJ |
| 003465 | BALB/c-Tg(CMV-cre)1Cgn/J |
| 013756 | C.129S-Batf3tm1Kmm/J |
| 004126 | C.Cg-Cd19tm1(cre)Cgn Ighb/J |
| 005673 | C.Cg-Tg(Mx1-cre)1Cgn/J |
| 006244 | C.Cg-Tg(tetO-cre)1Jaw/J |
| 009155 | C57BL/6-Cldn6tm1(cre)Dkwu/J |
| 035489 | C57BL/6-Elmo1tm1Mki/J |
| 017557 | C57BL/6-Tg(BEST1-cre)1Jdun/J |
| 016097 | C57BL/6-Tg(Car1-cre)5Flt/J |
| 026828 | C57BL/6-Tg(Cpa3-cre)4Glli/J |
| 008870 | C57BL/6-Tg(Hspa2-cre)1Eddy/J |
| 024034 | C57BL/6-Tg(Pmch-cre)1Rck/J |
| 034448 | C57BL/6J-Tlr9em1Ldm/J |
| 030379 | C57BL/6J-Tg(Abcb1a-cre)NIDA045Htz/J |
| 032080 | C57BL/6J-Tg(Cd8a*-cre)B8Asin/J |
| 018899 | C57BL/6J-Tg(Krt6,-cre,-Cerulean)5Grsr/Grsr |
| 021582 | C57BL/6J-Tg(Mchr1-cre)1Emf/J |
| 022887 | C57BL/6J-Tg(Six6-cre)7Grsr/GrsrJ |
| 023453 | C57BL/6N-Lhx8tm1(cre,Cerulean)Grsr/2GrsrJ |
| 030484 | C57BL/6N-Tg(Oxr1-cre)C14Stl/J |
| 012686 | C57BL/6N-Tg(Ppp1r2-cre)4127Nkza/J |
| 028325 | C57BL/6NJ-Cldn13em2J/J |
| 026266 | D2.B6-Tg(Zp3-cre)93Knw/SjJ |
| 026852 | D2.Cg-Tg(Gfap-cre)73.12Mvs/SjJ |
| 026860 | D2.FVB-Tg(Tek-cre)2352Rwng/SjJ |
| 004600 | FVB-Tg(GFAP-cre)25Mes/J |
| 006139 | FVB.Cg-Tg(ACTA1-cre)79Jme/J |
| 017595 | FVB.Cg-Tg(CAG-cre/Esr1*)5Amc/J |
| 006297 | FVB.Cg-Tg(Eno2-cre)39Jme/J |
| 008244 | FVB.Cg-Tg(tetO-cre)1Jaw/J |
| 025066 | FVB/N-Tg(Mylpf-cre)3Kraj/Mmjax |
| 003377 | FVB/N-Tg(Zp3-cre)3Mrt/J |
| 017678 | FVB;129-Pink1tm1Aub Tg(Prnp-SNCA*A53T)AAub/J |
| 023325 | FVB;B6-Tg(Pbsn-cre)20Fwan/J |
| 034859 | NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CAG-cre/Esr1*)5Amc/J |
| 034795 | NOD.Cg-Tg(CAG-cre/Esr1*)5Amc/J |
| 013251 | NOD.FVB-Tg(EIIa-cre)C5379Lmgd/J |
| 004986 | NOD/ShiLt-Tg(Ins2-cre)3Lt/LtJ |
| 003855 | NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ |
| 035185 | STOCK Adgrl1tm2c(EUCOMM)Hmgu/SudJ |
| 012899 | STOCK Agrptm1(cre)Lowl/J |
| 025665 | STOCK Asxl1tm1.1Iaai/J |
| 018473 | STOCK Egfrtm1.1Tyj/J |
| 007916 | STOCK En1tm2(cre)Wrst/J |
| 026199 | STOCK F8tm1.1Rmnt/J |
| 027012 | STOCK Foxj1tm1.1(cre/ERT2/GFP)Htg/J |
| 007913 | STOCK Gli1tm3(cre/ERT2)Alj/J |
| 008783 | STOCK Grm7Tg(SMN2)89Ahmb Smn1tm3(SMN2/Smn1)Mrph Tg(CAG-cre/Esr1*)5Amc Tg(SMN2*delta7)4299Ahmb/J |
| 022793 | STOCK Gt(ROSA)26Sortm1(LRRK2*R1441C)Djmo/J |
| 024283 | STOCK Hcn4tm2.1(cre/ERT2)Sev/J |
| 017298 | STOCK Hmgn5tm1.1Mbus/J |
| 032429 | STOCK Krastm4Tyj Trp53tm1Brn Tg(Pdx1-cre/Esr1*)#Dam/J |
| 018418 | STOCK Lrig1tm1.1(cre/ERT2)Rjc/J |
| 016945 | STOCK Mir138-1tm1Mtm/Mmjax |
| 004192 | STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J |
| 025669 | STOCK Myf6tm1.1(cre/ERT2)Lepr/J |
| 030291 | STOCK Ntrk3tm3.1(cre/ERT2)Ddg/J |
| 035045 | STOCK Oprk1tm1.1(cre)Sros/J |
| 030543 | STOCK Oxtrtm1(cre/GFP)Rpa/J |
| 022972 | STOCK Pros1tm1Grl/J |
| 023329 | STOCK Ptf1atm1(cre)Hnak/RschJ |
| 019378 | STOCK Ptf1atm2(cre/ESR1)Cvw/J |
| 013086 | STOCK Rxratm1Krc/J |
| 020080 | STOCK Slc6a3tm1(cre)Xz/J |
| 023405 | STOCK Tfap2atm1Hsv/J |
| 024849 | STOCK Et(cre)TFC.09Lois/J |
| 025750 | STOCK Tg(ACTA1-cre/Esr1*)2Kesr/J |
| 027826 | STOCK Tg(Actl6b-Cre)4092Jiwu/J |
| 007684 | STOCK Tg(Atoh1-cre/Esr1*)14Fsh/J |
| 032027 | STOCK Tg(Avil-icre/ERT2)AJwo/J |
| 032910 | STOCK Tg(BEST1-rtTA,tetO-cre)1Yzl/J |
| 004453 | STOCK Tg(CAG-cre/Esr1*)5Amc/J |
| 035566 | STOCK Tg(CAG-mRuby2*,-mClover*)1Srgt/J |
| 009615 | STOCK Tg(Cartpt-cre)1Aibs/J |
| 017336 | STOCK Tg(Cd4-cre)1Cwi/BfluJ |
| 025736 | STOCK Tg(Cela1-cre/ERT)1Lgdn/J |
| 005105 | STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J |
| 031049 | STOCK Tg(Colgalt2-cre)NF111Htz/J |
| 008852 | STOCK Tg(En2-cre)22Alj/J |
| 005938 | STOCK Tg(Eno2-cre)39Jme/J |
| 022763 | STOCK Tg(Eno2-cre/ERT2)1Pohlk/J |
| 026625 | STOCK Tg(Gcm1-cre)1Chrn/J |
| 029485 | STOCK Tg(Gsx1-cre)6Wql/J |
| 019380 | STOCK Tg(IVL-cre/ERT2)1Blpn/J |
| 029417 | STOCK Tg(Jam2-cre/ERT2)2Jrs/J |
| 008582 | STOCK Tg(Kcnc2-Cre)K128Stl/LetJ |
| 017948 | STOCK Tg(KRT18-cre/ERT2)23Blpn/J |
| 023724 | STOCK Tg(mI56i-cre,EGFP)1Kc/J |
| 003553 | STOCK Tg(MMTV-cre)4Mam/J |
| 008847 | STOCK Tg(Msx2-cre/ERT)27Alj/J |
| 009074 | STOCK Tg(Myh6-cre)1Jmk/J |
| 009102 | STOCK Tg(Nefh-cre)12Kul/J |
| 005667 | STOCK Tg(Neurog3-cre)C1Able/J |
| 006207 | STOCK Tg(Pcp2-cre)1Amc/J |
| 009606 | STOCK Tg(Six2-EGFP/cre)1Amc/J |
| 032646 | STOCK Tg(Spo11-cre)1Rsw/PecoJ |
| 006224 | STOCK Tg(tetO-cre)1Jaw/J |
| 008851 | STOCK Tg(Wnt1-cre/ERT)1Alj/J |
| 018281 | STOCK Tg(Wnt7a-EGFP/cre)#Bhr/Mmjax |
| 233 strains use this protocol | |