Fewer than expected homozygotes are produced from heterozygote crosses (with even fewer female homozygotes produced). This from IMR Database.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
HET = 700 bp and 548 bp
MUT = 700 bp
WT = 548 bp
Separated by gel electrophoresis on a 1.5% agarose gel.