***Use Perfect Match® (Stratagene) added before Taq (1:1) to delete non-specific bands
This assay will NOT distinguish hemizygous from homozygous transgenic animals.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Transgene = 246 bp
Internal positive control = 156 bp
Separated by gel electrophoresis on a 1.5% agarose gel.