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Mutant = -/-
Heterozygote = G/-
Wild type = G/G
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
p116 | AAC ATC CAC GTA CGG | |||||
poIMR0114 | Fluorophore | GTG TGA GGG GTA AAT TCA CTC GAC | ||||
poIMR0115 | CAA ACT GGC TAT TCA GCT GTG G |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.00 |
MgCl2 | 2.00 |
dNTPS-kapa | 0.20 |
poIMR0114 | 0.50 |
poIMR0115 | 0.50 |
Glycerol | 5.00 |
Kapa 2G HS taq polym | 0.01 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
006564 | B6(C)-KitW-41J Gusbmps/BrkJ |
006559 | B6.C-H2-Kbm1/ByBir-Gusbmps/BrkJ |
000256 | B6.C-H2-Kbm1/ByBir-Gusbmps/J |
006562 | B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ |
002086 | B6.Cg-Gusbmps Tg(Gussx)1Wat/J |
006407 | B6.Cg-Gusbmps/BrkJ |
005053 | NOD.Cg-Prkdcscid Gusbmps/SndsJ |
001880 | STOCK Gusbmps Tg(GUSB)4Sly/BirJ |
8 strains use this protocol |