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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR6214 | CCT GTC TCA TTT GCA TGT G | |||||
oIMR6215 | GAT AAC ATC CAC GTA CCG G |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 X |
MgCl2 | 2.00 mM |
dNTP | 0.20 mM |
oIMR6214 | 1.00 uM |
oIMR6215 | 1.00 uM |
DNA Loading Dye | 0.69 mM |
Taq DNA Polymerase | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 55.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold** |
Component | Final Concentration |
---|---|
AB PCR BufferII | X |
ddH2O | |
BSA | mg/ml |
NciI |
Stock Number | Strain Name |
---|---|
006564 | B6(C)-KitW-41J Gusbmps/BrkJ |
006559 | B6.C-H2-Kbm1/ByBir-Gusbmps/BrkJ |
000256 | B6.C-H2-Kbm1/ByBir-Gusbmps/J |
006562 | B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ |
002086 | B6.Cg-Gusbmps Tg(Gussx)1Wat/J |
006407 | B6.Cg-Gusbmps/BrkJ |
005053 | NOD.Cg-Prkdcscid Gusbmps/SndsJ |
001880 | STOCK Gusbmps Tg(GUSB)4Sly/BirJ |
8 strains use this protocol |