The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Alpha = 911 bp
Beta = 851 bp
Rxn A (primers 6006 and 6008) for Beta trangene (HLA-DQB1)
Rxn B (primers 6004 and 6005) for Alpha transgene (HLA-DQA1)
Use Perfect Match PCR Enhancer for best results.
Separated by gel electrophoresis on a 1.5% agarose gel.