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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
9746 | GCC AAG GTG AGG TGG ATA GA | Forward | ||||
9747 | TCC TCT GAT AGG GCC AAA GA | Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 |
MgCl2 | 2.00 |
dNTP | 0.20 |
9746 | 0.10 |
9747 | 0.10 |
DNA Loading Dye | 0.69 |
Taq DNA Polymerase | 0.03 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 62.0 | -- | |
4 | 72.0 | -- | |
5 | -- | Go to step 2-4, 35 times | |
6 | 72.0 | -- | |
7 | 10.0 | -- | hold |
Component | Final Concentration |
---|---|
ddH2O | |
NEB Buffer 3 | 1.00 |
AciI | 0.83 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 37.0 | -- |