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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR4352 | GAA GGT GAC CAA GTT CAT GCT GAG GAC GCT CTC TGC CAT T | |||||
oIMR4353 | GAA GGT CGG AGT CAA CGG ATT AGG ACG CTC TCT GCC ATC | |||||
oIMR4354 | ACC GCT TCC CAA CTA CTG AT |
Component | Final Concentration |
---|---|
ddH2O | |
Ampliflour SNP FAM | 1.00 X |
SNP JOE | 1.00 X |
Primer Mix | 1.00 X |
Rxn Mix S+ | 1.00 X |
dNTP | 0.20 mM |
Titanium Taq Pol. | 0.05 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 94.0 | -- | |
3 | 55.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 40 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold |