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Mutant GTC GTG GTG ACC
Wild type GTC ATT ATG ACC
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
17090 | CAC CCC ATT CAC AGA AGA CA | Forward | A | |||
17091 | CAA CCC ATA GGC AGG TCA AG | Reverse | A | |||
17092 | Fluorophore-1 | TGT CAT TGT CAT TAT GAC CAT CCT | Quencher-1 | Wild type | A | |
17093 | Fluorophore-2 | TCA TTG TCG TGG TGA CCA TC | Quencher-2 | Mutant | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
17090 | 0.40 uM |
17091 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
031988 | 129S4.Cg-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/LfaJ |
004193 | B6.129-Psen1tm1Mpm/J |
033930 | B6.Cg-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/2J |
008880 | B6.Cg-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/J |
004807 | B6;129-Tg(APPSwe,tauP301L)1Lfa Psen1tm1Mpm/Mmjax |
5 strains use this protocol |