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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
9231 | GGA GCG GAG TAC ATG CAA TC | |||||
9232 | Fluorophore | TCG TCC ACT GCC TTA GTA TCC | ||||
9233 | GGA AGT GCT CAA CCT C |
Component | Final Concentration |
---|---|
ddH2O | |
KAPA LR HotStart Buf Buffer does not contain MgCl2. | 1.00 |
MgCl2 | 2.00 |
dNTP | 0.13 |
KAPA LR HotStart Taq Initial Denature 3 Min This is a two enzyme system. One with proofreeading the other without. HotStart is provided by antibody. | 0.02 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 60.0 | -- | |
4 | 65.0 | -- | |
5 | -- | Go to step 2-4, 50 times | |
6 | 65.0 | -- | |
7 | 10.0 | -- | hold |