For in-depth product & services help, ask our
Technical Information Scientists
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR1436 | GGT AAA GAC CCA TGT GAC CC | Common | ||||
oIMR1437 | TCC ACC TAG CCT GCC TGT AC | Mutant | ||||
oIMR1438 | GGC TTG CCA CAT GAC AA | Wild type |
Component | Final Concentration |
---|---|
ddH2O | |
High Resolution Melt | 1.00 X |
MgCl2 | 2.00 mM |
oIMR1436 | 1.00 uM |
oIMR1437 | 1.00 uM |
oIMR1438 | 1.00 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 59.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold |