SEQ results using DNA RESOURCE -/- indicates oIMR7430 is the common unless primer is labeled wrongly. (Each WT amplified separately with Mut primer) SC 8-14-13
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = 410 bp
Heterozygote = 225 bp and 410 bp
Wild type = 225 bp
Separated by gel electrophoresis on a 1.5% agarose gel.