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Transgene = 79 bp
Internal positive control = 74 bp
This assay can be used for tTA & rtTA but not rtTA Advanced(rtTA*M2)
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
13734 | Fluorophore-1 | CGC ATG CCG ACG CGC TAG | Quencher-1 | Tg Probe | ||
9342 | GGA CGA GCT CCA CTT AGA CG | Forward | A | |||
9343 | CAA CAT GTC CAG ATC GAA ATC | Reverse | A | |||
oIMR1544 | CAC GTG GGC TCC AGC ATT | Internal Positive Control Forward | A | |||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | Internal Positive Control Reverse | A | |||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 | IC Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
9342 | 0.40 uM |
9343 | 0.40 uM |
oIMR1544 | 0.40 uM |
oIMR3580 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
016198 | 129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ |
027783 | B6(Cg)-Tg(tetO/Prnp-GCaMP3)600Cai/J |
029476 | B6.Cg-Col1a1tm1(tetO-cas9)Sho/J |
026262 | B6.Cg-Igs7tm92.1(tetO-ECFP*/Venus*)Hze/J |
003563 | B6.Cg-Tg(Cebpb-tTA)5Bjd/J |
003767 | B6.Cg-Tg(Eno2tTA)5021Nes/J |
003763 | B6.Cg-Tg(Eno2tTA)5030Nes/J |
005964 | B6.Cg-Tg(GFAP-tTA)110Pop/J |
002618 | B6.Cg-Tg(MMTVtTA)1Mam/J |
006232 | B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J |
028295 | B6.Cg-Tg(tetO-DRD3)3-10Kndl/J |
034533 | B6.FVB-Tg(EmuSR-tTa)83Bop/DwfJ |
012433 | B6;C3-Tg(ACTA1-rtTA,tetO-cre)102Monk/J |
007678 | B6;SJL-Tg(KRT14-rtTA)208Jek/J |
006242 | C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J |
006245 | C.Cg-Tg(SFTPC-rtTA)5Jaw/J |
013585 | FVB-Tg(Cdh5-tTA)D5Lbjn/J |
005625 | FVB-Tg(Pcp2-tTA)3Horr/J |
006222 | FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J |
006225 | FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J |
006209 | FVB.Cg-Tg(Tal1-tTA)19Dgt/J |
034802 | FVB/N-Tg(MMTV-tTA)25754Kuw/Mmjax |
004127 | FVB/N-Tg(Nes-rtTA)306Rvs/J |
005942 | FVB/N-Tg(Pf4-tTA/VP16)42Kra/J |
006875 | FVB/N-Tg(Tagln-rtTA)E1Jwst/J |
004937 | NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ |
005734 | NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ |
006999 | STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J |
018999 | STOCK Gt(ROSA)26Sortm1(tTA,tetO-Mir155)Fjsl/J |
024108 | STOCK Igs7tm93.1(tetO-GCaMP6f)Hze Tg(Camk2a-tTA)1Mmay/J |
024854 | STOCK Tg(Camk2a-tTA)1Mmay Fgf14Tg(tetO-MAPT*P301L)4510Kha/J |
003272 | STOCK Tg(Cebpb-tTA)5Bjd/J |
003273 | STOCK Tg(CMV-rtTA)4Bjd/J |
003271 | STOCK Tg(CMV-tTA)3Bjd/J |
005493 | STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J |
008790 | STOCK Tg(tetO-DISC1*)1001Plet/J |
36 strains use this protocol |