Protocol 31676: Separated PCR Assay - GAL Panel-B6J vs. B6N

B6;SJL-Tg(CD8aCD8b)57Scr/J

Stock No: 003202

Version 1.0

Notes

The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.

Expected Results

JAX Protocol

Protocol Primers

Primer	5' Label	Sequence 5' → 3'	3' Label	Primer Type	Reaction	Note

Strains Using This Protocol

Stock Number	Strain Name
010611	B6.129P2(Cg)-Ighg1^tm1(cre)Cgn/J
023337	B6.129S4-Cxcr3^tm1Arsa/SoghJ
017009	B6.129X1-Nfe2l2^tm1Ywk/J
003202	B6;SJL-Tg(CD8aCD8b)57Scr/J
4 strains use this protocol