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Mutant = X bp
Heterozygote = X bp and Y bp
Wild type = Y bp
Converting to 12ul, email genotyping@jax.org with jpeg
Mutant = X bp
Heterozygote = X bp and Y bp
Wild type = Y bp
gttgtctaatagaggtaactggactgaaacaatatttctgtttgtttattctttctttttcttatagAACATATTTCTAACCAAAGATGGGgACAGTGCAGCTTGGAGATTTTGGAATTGCTCG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
16868 | CGA GCA ATT CCA AAA TCT CC | Reverse | A | |||
16869 | TTG TCT AAT AGA GGT AAC TGG ACT GA | Forward | A | |||
16870 | Fluorophore-1 | AAA GAT GGG GAC AGT GCA G | Quencher-1 | MUT Probe | ||
16871 | Fluorophore-2 | AAA GAT GGG ACA GTG CAG | Quencher-2 | WT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
16868 | 0.40 uM |
16869 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |