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Transgene = 58 bp
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
12485 | GCC CAA GTT GGG AAG ACA AC | Transgene Forward | ||||
12486 | CCA GCT TGG TTC CCG AAT AGA C | Transgene Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 |
MgCl2 | 2.00 |
dNTP | 0.20 |
0.00 | |
0.00 | |
0.00 | |
0.00 | |
DNA Loading Dye | 0.69 |
Taq DNA Polymerase | 0.03 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | -- | ||
4 | 72.0 | -- | |
5 | -- | Go to step 2-4, 35 times | |
6 | 72.0 | -- | |
7 | 10.0 | -- | hold |