The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = 464 bp
Heterozygote = 175 bp and 464 bp
Wild type = 175 bp
Separated by gel electrophoresis on a 1.5% agarose gel. Do not pool pcr products.