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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR0810 | TGG TTC TGG AAT GAG GAT GG | Forward | ||||
oIMR0811 | TGC CTC TGC TTC CTT AGT GC | Reverse |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 0.89 X |
MgCl2 | 1.33 mM |
dNTP | 0.18 mM |
oIMR0810 | 1.19 uM |
oIMR0811 | 1.19 uM |
DNA Loading Dye | 0.62 mM |
Perfect Match (Strat | 0.07 U/ul |
Taq DNA Polymerase | 0.02 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold** |
Component | Final Concentration |
---|---|
AB PCR BufferII | X |
ddH2O | |
BSA | mg/ml |
Sph I |