For in-depth product & services help, ask our
Technical Information Scientists
Mutant = A/A
Heterozygote = C/A
Wild type = C/C
This strain is x-linked
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
18357 | GGA GGA CAC CCT TCC TTT CT | Forward | A | |||
18358 | AGC GGT GTC TGT GAG ACT TTC | Reverse | A | |||
18359 | Fluorophore-1 | CAC AAG ACA TTC ACT TGG GTG TG | Quencher-1 | Wild type | A | |
18360 | Fluorophore-2 | CAC AAG ACA TTA ACT TGG GTG TG | Quencher-2 | MUT Probe | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
18357 | 0.40 uM |
18358 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |