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Taqman qPCR protocols are run on a real time PCR instrument. Use an appropriate instrument specific Fluorophore/Quencher combination. The transgene genotype is determined by comparing ΔCt values of each unknown sample against known homozygous and hemizygous controls, using appropriate endogenous references.
Tg= 84 bp
IPC = 74 bp
This assay was not tested on homozygotes so it may or may not be able to distinguish hemi from hom
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
52551 | CCT GCG GCT AAG TGA GAT GT | Transgene Forward | Apoa2 | |||
52552 | AGG TAG GGA GAG GGG CTC TA | Transgene Reverse | Apoa2 | |||
52553 | Fluorophore-1 | CCA GGC CCA GTC TTC CCA C | Quencher-1 | Tg Probe | ||
oIMR1544 | CAC GTG GGC TCC AGC ATT | |||||
oIMR3580 | TCA CCA GTC ATT TCT GCC TTT G | |||||
TmoIMR0105 | Fluorophore-2 | CCA ATG GTC GGG CAC TGC TCA A | Quencher-2 |