For in-depth product & services help, ask our
Technical Information Scientists
Mutant = T/T, T/T, -/-
Heterozygote = C/T, C/T, G/-
Wild type = C/C, C/C, G/G
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
14996 | TAT GGA GCG TCC TCA CTT CC | Forward | A | |||
14997 | GAC AAG TTC TGG CGT TCT CC | Reverse | A | |||
14998 | Fluorophore-1 | GGG CCT TGG CCT GCT C | Quencher-1 | WT Probe | ||
14999 | Fluorophore-2 | ATG GGT CTT GGC TTC TCG | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
14996 | 0.40 uM |
14997 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |