This assay tests for the presence of the retinal degeneration Pde6brd1 mutation.
The WT-specific primer, oIMR2094, sits on a known SNP identified as rs47440869, located at the nucleotide indicated in red: ACC TGC ATG TGA ACC CAG TAT TCT ATC. Information can be found at http://www.informatics.jax.org/snp/rs47440869.
The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = 560 bp
Heterozygote = 240 bp and 560 bp
Wild type= 240 bp
Separated by gel electrophoresis on a 1.5% agarose gel.