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Mutant =-/-
Heterozygote = C/-
Wild type = C/C
chr5:129993523-129993623 101bp GTAAATTCACTCGACCGCTCTT CACCTCCAAATGCCCATAGT
The Fwd primer (primer 56143) anneals over the nucleotide sequence containing mouse genomic variations rs48872564.
The WT & Mut probes anneal over the nucleotide sequence containing mouse genomic variation rs48332902
cgaccgctcttgtcttagGCCC(C/-)GTACGTGGATGTTATCTGTGTAAACAGCTACTTTTCTTGGTATCATGAC
TATGGGCATTTGGAGGTGATTCAGCCACAGCTGAATAGCCAGTTTGAGAACTGGTATA
AGACGCATCAGAAGCCGATTATCCAGAGCGAGTATGGAGCAGACGCAATCCCAGGGATCCACGAG
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
56143 | GTA AAT TCA CTC GAC CGC TCT T | Forward | A | |||
56144 | CAC CTC CAA ATG CCC ATA GT | Reverse | A | |||
56145 | Fluorophore-1 | TCT TAG GCC CCG TAC GTG GAT | Quencher-1 | WT Probe | ||
56929 | Fluorophore-2 | CTT AGG CCC GTA CGT GGA | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
56143 | 0.40 uM |
56144 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
006564 | B6(C)-KitW-41J Gusbmps/BrkJ |
006559 | B6.C-H2-Kbm1/ByBir-Gusbmps/BrkJ |
000256 | B6.C-H2-Kbm1/ByBir-Gusbmps/J |
006562 | B6.CBy(Cg)-Gusbmps Gpi1a-m1J/BrkJ |
002086 | B6.Cg-Gusbmps Tg(Gussx)1Wat/J |
006407 | B6.Cg-Gusbmps/BrkJ |
005053 | NOD.Cg-Prkdcscid Gusbmps/SndsJ |
001880 | STOCK Gusbmps Tg(GUSB)4Sly/BirJ |
8 strains use this protocol |