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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR5211 | GAC TAC CCA AGT CCC CAT AGC | Reverse | ||||
oIMR5212 | GGA GGT TAC GCC AAA GGA TT | Forward |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.00 X |
MgCl2 | 2.00 mM |
dNTP | 0.20 mM |
oIMR5211 | 1.00 uM |
oIMR5212 | 1.00 uM |
DNA Loading Dye | 0.69 mM |
Taq DNA Polymerase | 0.03 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 58.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold |
Component | Final Concentration |
---|---|
AB PCR BufferII | 1.65 X |
ddH2O | |
Mwo I |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 60.0 | -- |