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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
16073 | CTC TCC AAC CAG CCC TAT ATC | Forward | A | |||
16074 | GAA CCA GTC TCA CCT CTA TAC AC | Reverse | A | |||
16075 | Fluorophore-1 | GTG AGC TTC AAG TCT CGG G | Quencher-1 | WT Probe | ||
16076 | Fluorophore-2 | GTG AGC TTC GAG TCT CGG G | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
16073 | 0.40 uM |
16074 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
037115 | B6(Cg)-Kmt2cem1Mgee/J |
001317 | B6.Cg-Gpi1a Thy1a Igha/J |
034140 | B6.Cg-Il17a/Il17ftm1.1Impr Thy1a/J |
038742 | B6.Cg-Thy1a Rbm5tm1.1Ozg/PkocJ |
035728 | B6.Cg-Thy1a Tg(CD2-Tcra,-Tcrb)27Glkm/J |
005023 | B6.Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J |
000406 | B6.PL-Thy1a/CyJ |
025854 | B6;FVB-Ptprca Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J |
017351 | BKa.Cg-Ptprcb Bmi1tm1Ilw Thy1a/J |
007686 | BKa.Cg-Sox17tm2Sjm Ptprcb Thy1a/J |
036333 | C.Cg-Rag2tm1Fwa Thy1a/AkhoAbhmJ |
036332 | C.Cg-Thy1a Tg(DO11.10)10Dlo/AkhoAbhmJ |
005307 | CBy.Cg-Thy1a Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ |
005443 | CBy.PL(B6)-Thy1a/ScrJ |
024476 | NOD.Cg-Stat4tm1Gru Thy1a Ifngr1tm1Agt Tg(TcraBDC2.5,TcrbBDC2.5)1Doi/LmbrJ |
004483 | NOD.NON-Thy1a/1LtJ |
005651 | SJL.AK-Thy1a/TseJ |
003961 | SJL.Cg Thy1a-Noxo1hslt/J |
025855 | STOCK Ptprca Lag3tm1Doi Tg(CAG-luc,-GFP)L2G85Chco Thy1a/J |
19 strains use this protocol |