For in-depth product & services help, ask our
Technical Information Scientists
ABI make a Custom SNP assay based on the sequence
MUT = A/A
HET = A/G
WT = G/G
Ctgggaatatggctgcctaagggagagggcctccctggaaagatgtgtgcagtatgtctcgtatgcagaaatacccactctgaccaggttgcccggggcctaggtctcccatcctttccttccggttgctctggagatcctaagagccctgaagctaaggcaaccacgtgcctgtcatctccctcagggagtacagtctgcctcctgtcttcaggacctgctataatgacctcttcatcttctctttgctccctagaggccattaactgtctgat[G/A]agagcaattgagatctatacagacatggtaagggttgcttgctgatcctgccttgctctgtcctctcctgtttatgctgtgctttagagggcagggatttgatcgctgagctgagctacagaacggggtgtgtgctgcagcctctgctcatgtcctcatgcacatgctgcctctgtccccttcgtgatccccttgtggctgcagtgtccatgcagtacttcccttgcctggccctcccctgatgtccctaggagcctgagaaatgctcagtctccccctctaggaaggctactatcctgagagacagc
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|
Component | Final Concentration |
---|---|
PerfeCTa qPCR SuperM UNG, ROX? | 1.00 X |
Assay Mix (ABI Custo | 1.04 X |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 92.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |