The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX).
To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility.
Reaction cycling temperature and times may require additional optimization based on the specific genotyping reagents used.
Mutant = ~110 bp and 58 bp
Heterozygote = 20 bp, 53 bp, 58 bp, 90 bp and ~110 bp
Wild type = 90 bp, 53 bp and 20 bp
Separate digested PCR product on a 5% agarose gel.**