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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
poIMR011 | GAT GAA CCC AAT CTA CCA ACT TCC | |||||
poIMR012 | Fluorophore | TGG CCA CAA AAT ATA CAC TTC TCT | ||||
poIMR013 | TTT ACA TTT TGA TGG AGG |
Component | Final Concentration |
---|---|
ddH2O | |
Kapa 2G HS buffer | 1.00 |
MgCl2 | 2.00 |
dNTPS-kapa | 0.20 |
poIMR011 | 0.50 |
poIMR012 | 0.50 |
Glycerol | 5.00 |
Kapa 2G HS taq polym | 0.01 |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 65.0 | -- | -0.5 C per cycle decrease |
4 | 68.0 | -- | |
5 | -- | repeat steps 2-4 for 10 cycles | |
6 | 94.0 | -- | |
7 | 60.0 | -- | |
8 | 72.0 | -- | |
9 | -- | repeat steps 6-8 for 28 cycles | |
10 | 72.0 | -- | |
11 | 10.0 | -- | hold |
Stock Number | Strain Name |
---|---|
002048 | B6 x C57BLKS-Dock7m Leprdb Myo15sh2-J/J |
000699 | B6.Cg-Dock7m Leprdb/+ +/J |
005089 | B6.Cg-Qkiqk-2J/GrsrJ |
000642 | BKS.Cg-Dock7m +/+ Leprdb/J |
000700 | BKS.Cg-Dock7m Leprdb/+ +/J |
008340 | BKS.Cg-Leprdb Nos3tm1Unc/RhrsJ |
001192 | BKS.Cg-meaJ Leprdb +/+ + Dock7m/J |
000707 | CBA.Cg-Dock7m Leprdb/+ +/J |
006654 | FVB.BKS(D)-Leprdb/ChuaJ |
9 strains use this protocol |