For in-depth product & services help, ask our
Technical Information Scientists
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|
Component | Final Concentration |
---|---|
Reaction Mix | 0.98 X |
Assay Mix | |
MgCl2 | 0.39 mM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 57.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 20 cycles |
5 | 94.0 | -- | |
6 | 57.0 | -- | |
7 | 72.0 | -- | repeat steps 5-7 for 20 cycles |
Component | Final Concentration |
---|---|
ddH2O |