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H2-T18<a> = C (Jrs 469, 4804, 465) = "hom"
H2-T18<b>= T (Jrs 666, 664, 457, 438) = "wt"
Tctgatgagttattgatctagctatgataa(T/C)cagtgattg(ATTG/----)ctattat(A/C)acagtcaca
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
25550 | Fluorophore-1 | AAT CAG TGA TTG ATT GCT ATT ATA AC | Quencher-1 | WT Probe | A | |
25551 | Fluorophore-2 | TAA CCA GTG ATT GCT ATT ATC ACA G | Quencher-2 | MUT Probe | A | |
25833 | AGT CCA AAG TTT TCA CAT TAA TCA | Forward | A | |||
25834 | TCA ATG AGA AGC CCT TTT AAG TC | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
25550 | 0.40 uM |
25551 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
000469 | B10.A-H2a H2-T18a/SgSnJ |
000465 | B10.BR-H2k2 H2-T18a/SgSnJ |
004804 | B10.BR-H2k2 H2-T18a/SgSnJJrep |
036602 | B10.Cg-H2k2 H2-T18a Tg(SV40-HA)HA104Ajca/VradMmjax |
037369 | STOCK H2u H2-T18a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ |
037372 | STOCK Il10tm1Flv H2u H2-T18a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ |
037371 | STOCK Ptprca Pepcb H2u H2-T18a Tg(CD2-TcraAc1-9,-TcrbAc1-9)4Kio/WdcJ |
7 strains use this protocol |