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Mutant allele is Hc<0> and is a 2-base TA deletion. It is found in A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ. JR000461. HOM controls are available from DNA resource.
Wild type allele is Hc<1> and is found in BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. JR000463
also have a pyro assay that works well. KC 9-20-12
Mutant allele is Hc<0> and is a 2-base TA deletion. It is found in A/HeJ, AKR/J, DBA/2J, NZB/B1NJ, SWR/J, B10.D2/oSnJ. JR000461
Wild type allele is Hc<1> and is found in BALB/cJ, C57BL/6J, DBA/1J, and B10.D2/nSnJ. JR000463
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
15123 | TCC ACA GGT ATG GTG TTT GG | Forward | A | |||
15124 | CCA AAG GTA CTG CAA AAT CC | Reverse | A | |||
15125 | Fluorophore-1 | ACT GGA ACT GCA TAC TTT GAA ATT AA | Quencher-1 | WT Probe | ||
15126 | Fluorophore-2 | ACT GGA ACT GCA CTT TGA AAT TAA | Quencher-2 | MUT Probe |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
15123 | 0.40 uM |
15124 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | |
4 | -- | repeat steps 2-3 for 40 cycles | |
5 | 40.0 | -- | Forever |
Stock Number | Strain Name |
---|---|
027528 | B10.Cg-H2d Tg(Alb1-HBV)66Bri/ChiJ |
003147 | B10.Cg-Hc1 H2d H2-T18c Tg(DO11.10)10Dlo/J |
000460 | B10.D2-Hc0 H2d H2-T18c/o2SnJ |
000461 | B10.D2-Hc0 H2d H2-T18c/oSnJ |
000463 | B10.D2-Hc1 H2d H2-T18c/nSnJ |
034608 | J:ARC(S) |
004306 | NOD.CBALs-Hc1/LtJ |
030511 | NOD.Cg-Hc1 Prkdcscid Il2rgtm1Wjl/SzJ |
8 strains use this protocol |