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AAGCAGACACAGGAGAGAGGATGTTCTGTaaaacaagcatgtga
Aaggatgcg(A/-)tg(CGTG/-----)atgaaggattctttgctaaaGACACA
ATTATGGATCTGCAA
Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
21943 | Fluorophore-1 | ATG CGA TGC GTG ATG AAG | Quencher-1 | MUT Probe | ||
21944 | Fluorophore-2 | AAG GAT GCG TGA TGA AGG AT | Quencher-2 | WT Probe | ||
22205 | CAC AGG AGA GAG GAT GTT CTG | Forward | A | |||
22206 | AGG GTT GCA GAT CCA TAA TTG | Reverse | A |
Component | Final Concentration |
---|---|
Kapa Probe Fast QPCR | 1.00 X |
ddH2O | |
22205 | 0.40 uM |
22206 | 0.40 uM |
Wt Probe | 0.15 uM |
Mutant Probe | 0.15 uM |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 95.0 | -- | |
2 | 95.0 | -- | |
3 | 60.0 | -- | repeat steps 2-3 for 40 cycles |
Stock Number | Strain Name |
---|---|
002845 | C.B-H2b Tg(H2-Dd)D8Gja/LilJ |
001952 | C.B10-H2b/LilMcdJ |
000438 | C3.SW-H2b/SnJ |
000666 | C57BL/10SnJ |
004353 | C57BL/6-Tg(UBC-GFP)30Scha/J |
000664 | C57BL/6J |
005304 | C57BL/6NJ |
007076 | CByJ.B6-Tg(UBC-GFP)30Scha H2b/J |
002591 | NOD.B10Sn-H2b/J |
9 strains use this protocol |