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Primer | 5' Label | Sequence 5' → 3' | 3' Label | Primer Type | Reaction | Note |
---|---|---|---|---|---|---|
oIMR0725 | TTC CCC AAG CAT GGT ATC TAG T | |||||
oIMR0744 | ATA CGT ACA GAT GGG ATC |
Component | Final Concentration |
---|---|
ddH2O | |
AB PCR BufferII | 1.09 X |
MgCl2 | 2.18 mM |
dNTP | 0.22 mM |
oIMR0725 | 0.55 uM |
oIMR0744 | 0.55 uM |
DNA Loading Dye | 0.76 mM |
Taq DNA Polymerase | 0.02 U/ul |
DNA |
Step | Temp °C | Time | Note |
---|---|---|---|
1 | 94.0 | -- | |
2 | 94.0 | -- | |
3 | 55.0 | -- | |
4 | 72.0 | -- | repeat steps 2-4 for 35 cycles |
5 | 72.0 | -- | |
6 | 10.0 | -- | hold** |
Component | Final Concentration |
---|---|
AB PCR BufferII | X |
ddH2O | |
NdeI | U/ul |