LOCUS       Exported               11468 bp DNA     linear   UNA 11-JAN-2023
DEFINITION  FUS RNA binding protein [Source:HGNC Symbol;Acc:HGNC:4010].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2023 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     gene ENSG00000089280
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
     gene            1..11468
                     /label=FUS
                     /note="gene ENSG00000089280
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..11465
                     /label=FUS-209
                     /note="primary transcript ENST00000566605
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 2..11468
                     /label=FUS-201
                     /note="primary transcript ENST00000254108"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 2..11465
                     /label=FUS-202
                     /note="primary transcript ENST00000380244"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 2..3213
                     /label=FUS-205
                     /note="primary transcript ENST00000487045
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 13..11465
                     /label=FUS-206
                     /note="primary transcript ENST00000487509
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 14..11438
                     /label=FUS-210
                     /note="primary transcript ENST00000568685"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(78..90,2261..2285,2376..2527,3721..3865,4072..4259,
                     4802..5042,6665..6699,8188..8220,8986..9089,9528..9657,
                     9903..10004,10138..10261,10605..10705,10826..10973,
                     11262..11301)
                     /codon_start=1
                     /label=FUS-201
                     /note="coding sequence ENSP00000254108"
                     /note="This feature has 15 segments:
                         1:    78 ..    90 / #993366 / ENSE00003903481
                         2:  2261 ..  2285 / #993366 / ENSE00003475065
                         3:  2376 ..  2527 / #993366 / ENSE00003630677
                         4:  3721 ..  3865 / #993366 / ENSE00003599547
                         5:  4072 ..  4259 / #993366 / ENSE00003537617
                         6:  4802 ..  5042 / #993366 / ENSE00001791812
                         7:  6665 ..  6699 / #993366 / ENSE00003521089
                         8:  8188 ..  8220 / #993366 / ENSE00003535451
                         9:  8986 ..  9089 / #993366 / ENSE00003524436
                        10:  9528 ..  9657 / #993366 / ENSE00003534484
                        11:  9903 .. 10004 / #993366 / ENSE00003653971
                        12: 10138 .. 10261 / #993366 / ENSE00003604625
                        13: 10605 .. 10705 / #993366 / ENSE00003494295
                        14: 10826 .. 10973 / #993366 / ENSE00003637572
                        15: 11262 .. 11301 / #993366 / ENSE00001310182"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSG
                     GGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDN
                     SDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVS
                     FDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGG
                     GGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGG
                     PGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEH
                     RQDRRERPY"
     CDS             join(78..90,2261..2285,2376..2527,3724..3865,4072..4259,
                     4802..5042,6665..6699,8188..8220,8986..9089,9528..9657,
                     9903..10004,10138..10261,10605..10705,10826..10973,
                     11262..11301)
                     /codon_start=1
                     /label=FUS-202
                     /note="coding sequence ENSP00000369594"
                     /note="This feature has 15 segments:
                         1:    78 ..    90 / #993366 / ENSE00003903481
                         2:  2261 ..  2285 / #993366 / ENSE00003475065
                         3:  2376 ..  2527 / #993366 / ENSE00003630677
                         4:  3724 ..  3865 / #993366 / ENSE00002579873
                         5:  4072 ..  4259 / #993366 / ENSE00003537617
                         6:  4802 ..  5042 / #993366 / ENSE00001791812
                         7:  6665 ..  6699 / #993366 / ENSE00003521089
                         8:  8188 ..  8220 / #993366 / ENSE00003535451
                         9:  8986 ..  9089 / #993366 / ENSE00003524436
                        10:  9528 ..  9657 / #993366 / ENSE00003534484
                        11:  9903 .. 10004 / #993366 / ENSE00003653971
                        12: 10138 .. 10261 / #993366 / ENSE00003604625
                        13: 10605 .. 10705 / #993366 / ENSE00003494295
                        14: 10826 .. 10973 / #993366 / ENSE00003637572
                        15: 11262 .. 11301 / #993366 / ENSE00003606339"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNSYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPA
                     PSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSS
                     SGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGG
                     GGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNS
                     DNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSF
                     DDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGG
                     GSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGP
                     GGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHR
                     QDRRERPY"
     CDS             join(78..90,2261..2285,2376..2527,3721..3865,4072..4259,
                     4802..5042,6665..6699,8188..8220,8983..9089,9528..9657,
                     9903..10004,10138..10261,10605..10705,10826..10973,
                     11262..11301)
                     /codon_start=1
                     /label=FUS-210
                     /note="coding sequence ENSP00000455282"
                     /note="This feature has 15 segments:
                         1:    78 ..    90 / #993366 / ENSE00002606723
                         2:  2261 ..  2285 / #993366 / ENSE00003475065
                         3:  2376 ..  2527 / #993366 / ENSE00003630677
                         4:  3721 ..  3865 / #993366 / ENSE00003599547
                         5:  4072 ..  4259 / #993366 / ENSE00003537617
                         6:  4802 ..  5042 / #993366 / ENSE00001791812
                         7:  6665 ..  6699 / #993366 / ENSE00003521089
                         8:  8188 ..  8220 / #993366 / ENSE00003535451
                         9:  8983 ..  9089 / #993366 / ENSE00002575933
                        10:  9528 ..  9657 / #993366 / ENSE00003534484
                        11:  9903 .. 10004 / #993366 / ENSE00003653971
                        12: 10138 .. 10261 / #993366 / ENSE00003604625
                        13: 10605 .. 10705 / #993366 / ENSE00003494295
                        14: 10826 .. 10973 / #993366 / ENSE00003637572
                        15: 11262 .. 11301 / #993366 / ENSE00001922809"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSG
                     GGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSAEQD
                     NSDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATV
                     SFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYG
                     GGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGG
                     GPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGE
                     HRQDRRERPY"
     gene            complement(2374..3148)
                     /note="gene ENSG00000260304
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript complement(2374..3148)
                     /note="primary transcript ENST00000564743
                     lncRNA"
                     /note="color: #a6acb3; direction: LEFT"
     prim_transcript 3413..4261
                     /label=FUS-207
                     /note="primary transcript ENST00000487974
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 4943..7108
                     /label=FUS-213
                     /note="primary transcript ENST00000570090
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 5362..10965
                     /label=FUS-203
                     /note="primary transcript ENST00000474990
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 8015..9983
                     /label=FUS-211
                     /note="primary transcript ENST00000568901
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 8330..9558
                     /label=FUS-208
                     /note="primary transcript ENST00000564766
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 10079..11468
                     /label=FUS-212
                     /note="primary transcript ENST00000569760
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 10236..11421
                     /label=FUS-204
                     /note="primary transcript ENST00000483853
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     10776..10800
                     /label=PCR Forward
                     /note="color: black; sequence: GGGAACATAGGGGAATGGGAATATG; 
                     added: 2023-01-12"
     misc_feature    11247..11346
                     /label=Donor Template WT -> SNV
                     /note="color: #00ffff"
     primer_bind     complement(11247..11346)
                     /label=Donor Tempate WT -> SNV
                     /note="color: black; sequence: 
                     GGTACAGGACAAAAAGCTGTTCCAGAACCTGGGGAGCCAGGCTAATTAATACGGCCTCT
                     CCCTGTGATCCTGTCTGTGCTCACCCCTGCAAAAAAAAAAA; added: 
                     2023-01-12"
     misc_feature    11265..11284
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     primer_bind     11265..11284
                     /label=gRNA Protospacer
                     /note="color: black; sequence: TGAGCACAGACAGGATCGCA; added:
                     2023-01-12"
     misc_feature    11282
                     /label=SNV
                     /note="WT = G
                     SNV = A"
                     /note="color: #ffcc00"
     misc_feature    11285..11287
                     /label=PAM
                     /note="color: #ffff00"
     primer_bind     complement(11377..11396)
                     /label=Sanger Sequencing
                     /note="color: black; sequence: CCTTGGGTGATCAGGAATTG; added:
                     2023-01-12"
     primer_bind     complement(11433..11457)
                     /label=PCR Reverse
                     /note="color: black; sequence: TGGTCACTTTTAATGGGAACCAGAG; 
                     added: 2023-01-12"
ORIGIN
        1 tgctcagtcc tccaggcgtc ggtactcagc ggtgttggaa cttcgttgct tgcttgcctg
       61 tgcgcgcgtg cgcggacatg gcctcaaacg gtaggtaagg gcgcgaggcg acggcggcgg
      121 cgcacccggc cgaggcctcc cagctgggct tttcgttttc agtgggaccg gggcggcgat
      181 cccgtgtggg attttttggc gcccctgtgg cgggaagccg cggagaagag taactggagg
      241 aggctggtgt cgccattttg tttcgctcct ctggccctcg cgcgcggggc gggaagtctt
      301 ttctttgcag tccgtttgct tggggtgggc gttgggaggg acgcttctta ggggtttgaa
      361 gcgtcaggtg agggtggaaa acgcccattc tccgtggcct cgcctccccc aactcccggc
      421 cccgcgctcg agcccgcttt gtcgcagtgc tgcatccggg cactcgcggc gcgcacgcgc
      481 tctgcgggcc cctccccctt cgcggcgcgg gtaccccttc cccgcctcgt gttggttcag
      541 ctttctgtcg cgagaccctt cgcggaagac tcggcggcgc gcgtccggtg tgagccttgt
      601 ccctcagtgg tccttcgcga atgggcggga tcgctccgtt cccgcctggg ttgccacgcg
      661 gctgggggcg gaggctcggg atcggggccg ccctctagct taacggtttg gcggcggtgg
      721 tcagggttcg accaacggac ttggggacgg cccgagagtt tttcccgcct aaatttcttt
      781 cttttttttt ttggagacac ggtcttcctc tgtcgcccag gctggagtgc agtggtgcga
      841 tctcagatca ctgcaacgtc cacctcctgg gttcaagtga ttcttctgcc tcagcctcct
      901 gagtagttgg gattataggc gcccgccacc acagcccggc taatttttgt gtttcttagt
      961 aggggcgggg tttcaccatg ttggtcaggc tggtctcgaa ctgctgacct caggtgatcc
     1021 gcccgcctgg gcctcccgaa gtgtcgggat tacagacgta agccaaccac gcctggtcta
     1081 aatttctttt ttctgagata gggacggaga agaacggccg cccgaggcca caccctctcc
     1141 tggtcctctt tcccttttcc tgcgggggaa gcgccgcgtt tcctgtgctg ggaggatgag
     1201 ttgatcttgt tcgtgtatct taagtggggc ttttcaaaag cgcttttgtt gctttttgtt
     1261 cgctggggga aggcagggta ggtgagaaaa cggaaccatc tggagtccca gggctgggga
     1321 ctcgagtacc tgttgacttt cgcctcctaa ggcgagcagt tgcatgatct ctgtcatttg
     1381 gggtccaagg gctcttttga ttctctggct ttgcactaaa aagcccactt catctccggg
     1441 attggtcaaa gagtgaagaa tggccttttt tgagactttc ttattctgtg ggtctgggct
     1501 gagagagcag aggccacaat ctgaacactg ttcgaattca aacctgtgcc actttggtag
     1561 ttctgcgacg ttgggagagt tagtctcttg actcctggcg ataatggctt tctcatagag
     1621 tggatgaaac gaatgcgcga tgttttagag caatcactga catgaattgg gcttgcagaa
     1681 aattgtggat gtccaccaag accttggttt ttccaatggt taaggcttct gggactctgt
     1741 agaaacttgc attttcttca ctttttcttt attgtaagaa cacttggctg ataacgccag
     1801 tttgttcctc ttctcaacca gtgtctcatg gaggaatttt atgccctttg ttgcaacatg
     1861 gtcctatttt tatttttttt tttgagatgg agtcttgctc tgttgccagg ctggagtgca
     1921 atggctcact gcaacctctg cctcccgggt tcaagcgatt ctcctgcttc acctcccgag
     1981 tagctgggat tacaggcgtg cgccaccatg cccggctaat tttttgtatt tttattagag
     2041 atggggcttc accatgttgg tcaggccagt ctcgaattcc tgacctcaag tgatccaccc
     2101 acctcggcct cccaaactgc tgggattaca ggcatgatcc accgtgcctg gcctacgtgg
     2161 tcctttttat tcatcagtgc ttgagttaag gaatttagct ttaattcaac tctttcagag
     2221 tggcagctga agataatgtg attgtatttt tcttttgcag attataccca acaagcaacc
     2281 caaaggtgag tgctattttt gggcttccag agtttgtaga gggcaagggt ggtcacgcca
     2341 tgttttctga tcacgctggt tttcctttta tttagctatg gggcctaccc cacccagccc
     2401 gggcagggct attcccagca gagcagtcag ccctacggac agcagagtta cagtggttat
     2461 agccagtcca cggacacttc aggctatggc cagagcagct attcttctta tggccagagc
     2521 cagaacagtg agtctttctc agcgggtcac ctcttcctac tctttctgaa tattgctttt
     2581 ctttttcttg ttttttggag acggagtctg gtcctgttgc ccaggctgga gtgcagtggt
     2641 gctgtctcag ctcactgcaa catcagccta ccgggttcaa acgattctcc tgcctcagcc
     2701 tcctgagtag ctgggattac aggtacctgc taccacgcct ggctaatttt gtgtttttag
     2761 tagagatggg gtttcaccgt gttggacagg ctggtctgga actcctgacc tcctgcctgc
     2821 cttgacctgc caaagtgctg ggattacagg cgtcagccac aatgccctga atgttgcttt
     2881 tcttaaacct gagcagcact gagatgttga aactgttcca tatttctttt ccgtgaaaca
     2941 gtgtataagt cttaaaactt tttgggatct gagtccttta cagggcattg tggcacacct
     3001 gtagtcccag ctactgagga ggctgaggcg ggaggatccc ttgaattcag gagtttgggg
     3061 ctgcagtgag ctatgatggt gcctgtgaac agccactgca ttccagcctg ggcagtgtgt
     3121 tgaggcccca tctcaaaaac ataaaaaaaa aaacaaaaaa caaaaatgtt tattggtttg
     3181 tgattctgtt tccatttatt ttctttggct tttaattttt ttgactcttc ttattttcca
     3241 tcagcatgaa agagagcata ttttctaaag gaagaaccag ttttaggcca attctgaaat
     3301 ggagaaaatg gttttgtttg aaaatgtatg aaatcatgtg atacataagg aggtgggatt
     3361 tgccccaagg tcctgaagtg taactaagaa aggtggttgt cctgtagata ctgcacgcac
     3421 agctgcatat tacagtgctg ttaacaggga tccttgggcc tgggtttaga gggtggtgct
     3481 ggagatggtg ttgggattgg cggggtgaaa ttggaactgt actaaagagt tggtagaagt
     3541 tgaagcatta aatttaggct ttgaaaggag ggtaactatc tttgcctatg agttgcaaca
     3601 tcactaacag cttctgagag gctggcttta tgagtatagg tattatgttt tctttaaccc
     3661 attccttaca ttttctcttt cctggtggct tttgtgactc cctttttctt atcctggtag
     3721 caggctatgg aactcagtca actccccagg gatatggctc gactggcggc tatggcagta
     3781 gccagagctc ccaatcgtct tacgggcagc agtcctccta ccctggctat ggccagcagc
     3841 cagctcccag cagcacctcg ggaaggtacg gtggtgttga tgtcggggaa ggcttgaaaa
     3901 gaggggtgaa ttgatgagga atgataaagg gaccagcagt aggagcagtt cagaggtgta
     3961 attggggtag gggagcctgt gttgggtaca gagaatggac tccactaaaa gtgaaaggaa
     4021 attgggggct atgctgggat tgtgattgtg ttttttgttt gttttcccta gttacggtag
     4081 cagttctcag agcagcagct atgggcagcc ccagagtggg agctacagcc agcagcctag
     4141 ctatggtgga cagcagcaaa gctatggaca gcagcaaagc tataatcccc ctcagggcta
     4201 tggacagcag aaccagtaca acagcagcag tggtggtgga ggtggaggtg gaggtggagg
     4261 tgagatgtct tcagctttgt ctgcagccca ttttcttttt cttttttttt ttttttttga
     4321 gacggagtct tgctctgtct ctgttgctga ggctggagtg cagtggcaca atctcggctc
     4381 actgcaagct ccgccttccg ggttcgcgcc agtctcctgc ctcagcctcc cgagtagctg
     4441 ggactacagg catccgccac cacgcccggc taattttttg tatttttagt agagacgggg
     4501 tttcaccatg ttagccagga tggtttcgat ctcctgacct tgtgatccgc ccgccttggc
     4561 ctcccaaagt gctgggatta caggcgtgag ccactgtgcc tggtgtctgc agcccatttt
     4621 ctataaggat ttgtattctc ctgttttagc ttaaaagagg gttcctgtct tgtttcctag
     4681 ctgtcttttt actttctttt gtccttcatt gcctggcact tgtcaaacct tttcaaacct
     4741 tttagtgcta ctttacaatc tttttgtttt ttttttttaa tcattctttc ttttctcaca
     4801 ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat
     4861 ggcaatcaag accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga
     4921 ggccgcggca ggggtggcag tggtggcggc ggcggcggcg gcggtggtgg ttacaaccgc
     4981 agcagtggtg gctatgaacc cagaggtcgt ggaggtggcc gtggaggcag aggtggcatg
     5041 gggtaggtgt ctcatgagcc agggagtatc tttggtgggg agtgtggagg attgcatgaa
     5101 tctccctgaa gccagtccct agtgcatggt ttagtattct tgttgtctag ggatctgtga
     5161 gggctttgat ttgggggcag tgactttctt tttacatccc cattttattt ttgtgagaac
     5221 ttgggagcct gaactcccat ccataccact gaatagagat tttgagtaat gatacttgtt
     5281 tccaaaaaaa aagaaaccat acatagatac gtatggattg gagtcattaa tatcctaggc
     5341 aagaaacatg gaagtgaaga cttctttctc tgcaagggaa accgatgatc ccactcctgg
     5401 gaaatagtag ggaaacttgg tatgtgtatt cccatgtgtc ctctagggag ttggtaatgg
     5461 ttaacctgac ttcagcttcc aggaattggc tactcttccc gttttctata gtcatttgaa
     5521 tccacgagct tgatttgcac taatttgacc gacattgatt ttgtgtgtga cttggtttat
     5581 ggggccagct gactgaagta agcagacctt ttgggcaaaa atatgctttg acagtggtct
     5641 cccacctatt tgttccactg tctgccttcc cctggttact taaaattcat cagcttgtcc
     5701 aactggacct tctttccttc ctgctgaagt tgatttgaag taaaacctta gatttgatgt
     5761 taaaacagtt gtcaaatctg ttggtaaata agatttgaag gaccctactc tgtctccctt
     5821 gaaaaagggg aggaatgtca gtgttactgt ttttggaaaa agtagatttt taaaccgagt
     5881 ttggaaatgg taagtatgca gaggtgggtg ggggcaatct caaaaacgtg caaaaatgag
     5941 gaaaacaaaa atgaggaaat gtgtgcgtgt gtttaatgca aaactttaaa aagaaaaaca
     6001 actgttatgt gactgttaac ttgctctgca ttttatgtgc cacaggtatg aaaggtgaca
     6061 ttgcaaaata ctccgctctt ctcgcagtgt agaaggggtg accccggggg ttgggggaga
     6121 tcaaaaacag ctcagtagtt aggacagagc ttagctaagt ttgtcttgct ttaaggggaa
     6181 gttgcctttg gttttgactt tttatggaat ggggttgggt ctgcttgctg ctttcaaagc
     6241 aaaaaccaca aaaatgtgtt caaggctacc ccagcctggt gtgaaatgtc ttctgggtaa
     6301 attggggtag ggtttttaaa ccaactactt ggttgtcaac cacttgcgac aagaggaaaa
     6361 aaaaacatct gctccatcgg aagaacgacc aaggaaaatg ggttattttt tttccagagg
     6421 aaatagataa cgtaaccttt taaagcaaaa tctttataaa ctgtgtctga gaaattgcac
     6481 acgtgtgtgt gacatgctca aaggtcagac aaggggtggt caggaaggga tgtattttag
     6541 tagccacttg tatctttttc caaaaacacc tacccatgtt tggggaatgt taaacaaaat
     6601 caaaaaacaa ccttttgtag ccgttggaag cttcatgtcc tttcttctaa cttgtcttct
     6661 ccagcggaag tgaccgtggt ggcttcaata aatttggtgg taagtgaaca gagtttccaa
     6721 aattcccaac tcccagcaat gctttgtctg attgttcatt tgcagatgtc ttagcgtgtt
     6781 aatttaaatg tcaaaggttt tgaggtgtcc agaaccacct ccagaaaggg gtagggtaga
     6841 atgccacctg ttgcctggtg tgtgctaacc tggagcaggt aggggtaaga ctcaatagtc
     6901 atcttttacc aaatgggttt gccccaggtt aataagaggg gtctagtagg ccttggactg
     6961 ggccgttgcc acacctggca cttagtgacc atcatcatga gaaactggag agtgcgtgct
     7021 ggaacacgtg gtgccatctt ggctttagga tccttttgat cgttgtgtcc aaggcttgtg
     7081 tgtgtgtgag tgtgtgggag acaactccga atgtttaatt ctggaagagg gatgtaacat
     7141 tgccctgagg atggtgaagt tggtatacat ttataaagta cggaatggtg tcaatgaatg
     7201 caattctatg tatatggact taactgagat gggcaaatag aaactagctc tgggaaggaa
     7261 catgtgcact acttcaagaa agattggaag catgtgtggc tcatgggaaa taaccaggtc
     7321 ttaaacagca caaactgaat tcgtggacca ggaaggtctt aaacagcaca aactgaattc
     7381 atggaaaaat gacaaatttg agaagtctcc cagtaagctg gaacttttct ggtttggtta
     7441 acaaaaggtt tcttgatttg tttcaagatt taaagccaaa ggtgtgggtt catgacttag
     7501 gtgtcattgc gtgtgggtac aatatttata tatggcgaat tcagataaac attggtcaaa
     7561 gatggtctct ggaaaaacaa aatagaggct gcattacgga aataagattt ctggtctgtt
     7621 ccctgggaca tgcttaaaaa atacaatagc tattatgtat ggtttttatt ttcatgtggt
     7681 ttcggggaaa caacacggtt ttaaggatgg tttctaaaga tgaaattaaa aattgttcca
     7741 caagggttaa gtgtctggtg gtaaagttgg gagaaactgg atggatgcac atcgcatggc
     7801 tggtggcgag cccatctctc ttctctcggg tgagagaacc gggccaagct gagttggttt
     7861 gttcacttta atgggtctcc gtttcccctg ccacctgtgc tgaggacatt tcccagcctg
     7921 agctggggga ggcagcattt gctgaagtgt ggagttgtct ctgtggagac tcaagttaca
     7981 gatcttaagg ggcctgccta gaattttctc ctctgggcag gcgacccagg aaagggtttg
     8041 gagtgaggct gtgagcactt acttgatatt ttacaagttt ggatttggtg ttaatttttt
     8101 tccttgtccg ttttttcctg ttgactaacg gctcatcttt tccttgtttt tgtttttttt
     8161 ttgttctttt tttccatgtc actaaaggcc ctcgggacca aggatcacgt catgactccg
     8221 gtgagttcac acgtggtggc atgaaaagag tggctaaagt ggtatcaaga ctgcctggat
     8281 gttctttgaa actattataa aaaggaaact gaaaaaaatg gggatagaga aggaagggag
     8341 ttaggtgtgt ccttagttag cagtgagaag tatttgttac gaagtatttc tcagaaatac
     8401 ctggcttgtg ggttccaccc ccagtgattt aggtctgaga ggaccctgaa aatctacctt
     8461 tctaacaagt ccccagtgat gctgatgcgt ctggaccaca ctcagatggt ttacagcagt
     8521 ggttctttca aaatgtggat catgtccaag ttggtgaaca aaactagtga aagacctgac
     8581 cacatgaagt aaagcattga actcctgttt aggttgtatt gatgtttgtg tactagattt
     8641 gaatgtaaaa tgggtttctt atttaattcg gggaccttca actgaaagtt gataaatact
     8701 gatggacttt tctgtgtggt cttgttgggc atttcactcc tgagccctgg tttccatact
     8761 gtatactggg tgttaacatg tttcaaagga taattgtcaa actgaatctg aaatttatca
     8821 gcatggctgg catataggga ctcaaaaggg atgtggattt ctttttagtt gtcttccata
     8881 aaccaaatga taccagttgc ttgatggata ctaggtgctt taggtttttt cctgtgtttt
     8941 ttattttacc ttttcacatt tgcattttct ctgttcaaca agcagaacag gataattcag
     9001 acaacaacac catctttgtg caaggcctgg gtgagaatgt tacaattgag tctgtggctg
     9061 attacttcaa gcagattggt attattaagg tacttgtgga gaggagtggg agctttctgt
     9121 cagtgttgta ggcttgtgga tttcacacat tagtaaaagc aagtctttaa tggttgccag
     9181 cagtaaaaac aagtcttagt ggttgttgcc agcttaattt gttgaggaaa gagccttagt
     9241 tactgttttc taaaagagaa gttctatctt aacacaaaaa gtataactta tcagagtacc
     9301 ctaaactctt gagatttgta ctctatagta acttttagtt ttatctttca atattggagt
     9361 gagagacagt tttctttaat ggaggtttac atgtgaggta ggaagaagta actgggaaga
     9421 ggggagctga agtttgggaa ttataaacct catgttctag aggaagaaga tggaaaggga
     9481 gtactgtagc ctttaaaatt gatgttacct cattttgctt tcttcagaca aacaagaaaa
     9541 cgggacagcc catgattaat ttgtacacag acagggaaac tggcaagctg aagggagagg
     9601 caacggtctc ttttgatgac ccaccttcag ctaaagcagc tattgactgg tttgatggta
     9661 tgtatgagaa ggctggcaga ggtggggctg gggatatagg gcagcaagcc ttaggaaaca
     9721 agccatagtt tgagggttct tttgagtctt ccaacactta ctttagctgc ggtttcaggt
     9781 agtctcattt ttgcttatgt gtcagcagat tataaaccat ttgagaaagg cacgcttctc
     9841 ttgtattttc ggattaatgt gtcttgcatt taaagtctgt tgatgatttt ttgtttctct
     9901 aggtaaagaa ttctccggaa atcctatcaa ggtctcattt gctactcgcc gggcagactt
     9961 taatcggggt ggtggcaatg gtcgtggagg ccgagggcga ggaggtgagg agctacctgc
    10021 tagtggtgca gaggggtaat ggggagagtg cagaagatgg taaaggcttg catggaatgg
    10081 gttagattta ccaaacttgg agagggagca gacccatact tggtctatct gcattaggac
    10141 ccatgggccg tggaggctat ggaggtggtg gcagtggtgg tggtggccga ggaggatttc
    10201 ccagtggagg tggtggcggt ggaggacagc agcgagctgg tgactggaag tgtcctaatc
    10261 cgtgagtgaa acttaatttt tttcttagtt ctcttgcatg cgtgctcttt gatatattgg
    10321 tactgaggta tgtgcgtgtt ttccaaagaa gtaaatgtca aggccacact gttggggtca
    10381 gatttagcca aaagcttacc taggtaaggt tgatgtaatg ggaaaggtaa tggattgggt
    10441 tcagtaatac tgatttttgt tcctgactct gagaagcaag ccgttttgtc tttctgaagc
    10501 ttcagtttcc tcactgtatc tctaaagtca ccgtagtttc ttcctagttc taggtcttgc
    10561 ctattcccca tcgctccaga ctgattgtct tcctttctcc ttagcacctg tgagaatatg
    10621 aacttctctt ggaggaatga atgcaaccag tgtaaggccc ctaaaccaga tggcccagga
    10681 gggggaccag gtggctctca catgggtaag aaaggcagac ctggtgctag ggagctggga
    10741 ccaaagaatc cttaattttt cagcggggag gctcggggaa cataggggaa tgggaatatg
    10801 atagatcttg tttcttttgt cctagggggt aactacgggg atgatcgtcg tggtggcaga
    10861 ggaggctatg atcgaggcgg ctaccggggc cgcggcgggg accgtggagg cttccgaggg
    10921 ggccggggtg gtggggacag aggtggcttt ggccctggca agatggattc caggtaagac
    10981 tttaaatcag aataaaaaag tagagcagtt gaacagaggc cataggataa cagggttttg
    11041 ttgagaaagt ggtttcattt tgagggctag gtggaaagac ctgaggttgt aaccagtagt
    11101 ggagagggaa ggaaaattaa ctcaggggga gtgaatctgt agacccactt gagataagat
    11161 actcgctggg ttaggtagga ggggcagata ggatatctag gcttggagag gctggtaact
    11221 caaatataat ggatacttaa tttttttttt tttttttgca ggggtgagca cagacaggat
    11281 cgcagggaga ggccgtatta attagcctgg ctccccaggt tctggaacag ctttttgtcc
    11341 tgtacccagt gttaccctcg ttattttgta accttccaat tcctgatcac ccaagggttt
    11401 ttttgtgtcg gactatgtaa ttgtaactat acctctggtt cccattaaaa gtgaccattt
    11461 tagttaaa
//
