LOCUS Exported 3603 bp DNA linear UNA 13-OCT-2022 DEFINITION apolipoprotein E [Source:HGNC Symbol;Acc:HGNC:613]. ACCESSION . VERSION . KEYWORDS . SOURCE Homo sapiens ORGANISM Homo sapiens REFERENCE 1 (bases 1 to 3603) AUTHORS Ensembl TITLE Direct Submission JOURNAL Exported Oct 13, 2022 from SnapGene 5.3.2 https://www.snapgene.com COMMENT gene ENSG00000130203 FEATURES Location/Qualifiers source 1..3603 /organism="Homo sapiens" /mol_type="genomic DNA" gene 1..3603 /label=APOE /note="gene ENSG00000130203 Protein coding" /note="color: #a6acb3; direction: RIGHT" prim_transcript 1..3154 /label=APOE-204 /note="primary transcript ENST00000446996" /note="color: #a6acb3; direction: RIGHT" prim_transcript 6..3603 /label=APOE-201 /note="primary transcript ENST00000252486" /note="color: #ffcc00; direction: RIGHT" prim_transcript 6..1536 /label=APOE-205 /note="primary transcript ENST00000485628 Retained intron" /note="color: #a6acb3; direction: RIGHT" prim_transcript 22..3235 /label=APOE-203 /note="primary transcript ENST00000434152" /note="color: #a6acb3; direction: RIGHT" CDS join(79..133,812..877,1970..2162,2743..3235) /codon_start=1 /label=APOE-203 /note="coding sequence ENSP00000413653" /note="This feature has 4 segments: 1: 79 .. 133 / #993366 / ENSE00001601606 2: 812 .. 877 / #993366 / ENSE00003463686 3: 1970 .. 2162 / #993366 / ENSE00000893952 4: 2743 .. 3235 / #993366 / ENSE00001700162" /translation="MSSGASRKSWDPGNPWPPDWPITGRKMKVLWAALLVTFLAGCQAK VEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQEL RALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYR GEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIR ERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRT" prim_transcript 570..3164 /label=APOE-202 /note="primary transcript ENST00000425718" /note="color: #a6acb3; direction: RIGHT" CDS join(835..877,1970..2162,2743..3460) /codon_start=1 /label=APOE-201 /note="coding sequence ENSP00000252486" /note="This feature has 3 segments: 1: 835 .. 877 / #993366 / ENSE00003577086 2: 1970 .. 2162 / #993366 / ENSE00000893952 3: 2743 .. 3460 / #993366 / ENSE00000893954" /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQ ERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKS WFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH" CDS join(835..877,1970..2162,2743..3164) /codon_start=1 /label=APOE-202 /note="coding sequence ENSP00000410423" /note="This feature has 3 segments: 1: 835 .. 877 / #993366 / ENSE00001620702 2: 1970 .. 2162 / #993366 / ENSE00000893952 3: 2743 .. 3164 / #993366 / ENSE00001599675" /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQ" CDS join(835..877,1970..2162,2743..3154) /codon_start=1 /label=APOE-204 /note="coding sequence ENSP00000413135" /note="This feature has 3 segments: 1: 835 .. 877 / #993366 / ENSE00001667751 2: 1970 .. 2162 / #993366 / ENSE00000893952 3: 2743 .. 3154 / #993366 / ENSE00001664168" /translation="MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWE LALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVA EETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLR KRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSL" primer_bind 2064..2088 /label=PCR Forward /note="color: black; sequence: GGCACTGGGTCGCTTTTGGGATTAC; added: 2022-10-14" primer_bind 2879..2896 /label=Sanger Sequencing /note="color: black; sequence: GACATGGAGGACGTGTGC; added: 2022-10-14" misc_feature 2998..3097 /label=Donor Template WT -> SNV /note="color: #00ffff" primer_bind complement(2998..3097) /label=Donor Template WT -> SNV /note="color: black; sequence: CTCGCGGATGGCGCTGAGGCCGCGCTCGGCGCCCTCGCGGGCCCCGGCCTGGTACACTG CCAGGCACTTCTGCAGGTCATCGGCATCGCGGAGGAGCCGC; added: 2022-10-14" misc_feature 3016..3035 /label=Protospacer Sequence /note="color: #ff0000" primer_bind 3016..3035 /label=gRNA Protospacer /note="color: black; sequence: CGATGACCTGCAGAAGCGCC; added: 2022-10-14" misc_feature 3032 /label=SNV /note="WT = C SNV = T" /note="color: #ff9900" misc_feature 3036..3038 /label=PAM /note="color: #ffff00" primer_bind complement(3447..3471) /label=PCR Reverse /note="color: black; sequence: GGCTTCGGCGTTCAGTGATTGTCGC; added: 2022-10-14" gene 3585..9178 /note="gene ENSG00000280087 TEC" /note="color: #a6acb3; direction: RIGHT" prim_transcript 3585..9178 /note="primary transcript ENST00000623895 TEC" /note="color: #a6acb3; direction: RIGHT" ORIGIN 1 gagtcctact cagccccagc ggaggtgaag gacgtccttc cccaggagcc ggtgagaagc 61 gcagtcgggg gcacggggat gagctcaggg gcctctagaa agagctggga ccctgggaac 121 ccctggcctc caggtagtct caggagagct actcggggtc gggcttgggg agaggaggag 181 cgggggtgag gcaagcagca ggggactgga cctgggaagg gctgggcagc agagacgacc 241 cgacccgcta gaaggtgggg tggggagagc agctggactg ggatgtaagc catagcagga 301 ctccacgagt tgtcactatc atttatcgag cacctactgg gtgtccccag tgtcctcaga 361 tctccataac tggggagcca ggggcagcga cacggtagct agccgtcgat tggagaactt 421 taaaatgagg actgaattag ctcataaatg gaacacggcg cttaactgtg aggttggagc 481 ttagaatgtg aagggagaat gaggaatgcg agactgggac tgagatggaa ccggcggtgg 541 ggagggggtg gggggatgga atttgaaccc cgggagagga agatggaatt ttctatggag 601 gccgacctgg ggatggggag ataagagaag accaggaggg agttaaatag ggaatgggtt 661 gggggcggct tggtaaatgt gctgggatta ggctgttgca gataatgcaa caaggcttgg 721 aaggctaacc tggggtgagg ccgggttggg gccgggctgg gggtgggagg agtcctcact 781 ggcggttgat tgacagtttc tccttcccca gactggccaa tcacaggcag gaagatgaag 841 gttctgtggg ctgcgttgct ggtcacattc ctggcaggta tgggggcggg gcttgctcgg 901 ttccccccgc tcctccccct ctcatcctca cctcaacctc ctggccccat tcaggcagac 961 cctgggcccc ctcttctgag gcttctgtgc tgcttcctgg ctctgaacag cgatttgacg 1021 ctctctgggc ctcggtttcc cccatccttg agataggagt tagaagttgt tttgttgttg 1081 ttgtttgttg ttgttgtttt gtttttttga gatgaagtct cgctctgtcg cccaggctgg 1141 agtgcagtgg cgggatctcg gctcactgca agctccgcct cccaggtcca cgccattctc 1201 ctgcctcagc ctcccaagta gctgggacta caggcacatg ccaccacacc cgactaactt 1261 ttttgtattt tcagtagaga cggggtttca ccatgttggc caggctggtc tggaactcct 1321 gacctcaggt gatctgcccg tttcgatctc ccaaagtgct gggattacag gcgtgagcca 1381 ccgcacctgg ctgggagtta gaggtttcta atgcattgca ggcagatagt gaataccaga 1441 cacggggcag ctgtgatctt tattctccat cacccccaca cagccctgcc tggggcacac 1501 aaggacactc aatacatgct tttccgctgg gcgcggtggc tcacccctgt aatcccagca 1561 ctttgggagg ccaaggtggg aggatcactt gagcccagga gttcaacacc agcctgggca 1621 acatagtgag accctgtctc tactaaaaat acaaaaatta gccaggcatg gtgccacaca 1681 cctgtgctct cagctactca ggaggctgag gcaggaggat cgcttgagcc cagaaggtca 1741 aggttgcagt gaaccatgtt caggccgctg cactccagcc tgggtgacag agcaagaccc 1801 tgtttataaa tacataatgc tttccaagtg attaaaccga ctcccccctc accctgccca 1861 ccatggctcc aaagaagcat ttgtggagca ccttctgtgt gcccctaggt actagatgcc 1921 tggacggggt cagaaggacc ctgacccacc ttgaacttgt tccacacagg atgccaggcc 1981 aaggtggagc aagcggtgga gacagagccg gagcccgagc tgcgccagca gaccgagtgg 2041 cagagcggcc agcgctggga actggcactg ggtcgctttt gggattacct gcgctgggtg 2101 cagacactgt ctgagcaggt gcaggaggag ctgctcagct cccaggtcac ccaggaactg 2161 aggtgagtgt ccccatcctg gcccttgacc ctcctggtgg gcggctatac ctccccaggt 2221 ccaggtttca ttctgcccct gtcgctaagt cttggggggc ctgggtctct gctggttcta 2281 gcttcctctt cccatttctg actcctggct ttagctctct ggaattctct ctctcagctt 2341 tgtctctctc tcttcccttc tgactcagtc tctcacactc gtcctggctc tgtctctgtc 2401 cttccctagc tcttttatat agagacagag agatggggtc tcactgtgtt gcccaggctg 2461 gtcttgaact tctgggctca agcgatcctc ccgcctcggc ctcccaaagt gctgggatta 2521 gaggcatgag ccaccttgcc cggcctccta gctccttctt cgtctctgcc tctgccctct 2581 gcatctgctc tctgcatctg tctctgtctc cttctctcgg cctctgcccc gttccttctc 2641 tccctcttgg gtctctctgg ctcatcccca tctcgcccgc cccatcccag cccttctccc 2701 cgcctcccac tgtgcgacac cctcccgccc tctcggccgc agggcgctga tggacgagac 2761 catgaaggag ttgaaggcct acaaatcgga actggaggaa caactgaccc cggtggcgga 2821 ggagacgcgg gcacggctgt ccaaggagct gcaggcggcg caggcccggc tgggcgcgga 2881 catggaggac gtgtgcggcc gcctggtgca gtaccgcggc gaggtgcagg ccatgctcgg 2941 ccagagcacc gaggagctgc gggtgcgcct cgcctcccac ctgcgcaagc tgcgtaagcg 3001 gctcctccgc gatgccgatg acctgcagaa gcgcctggca gtgtaccagg ccggggcccg 3061 cgagggcgcc gagcgcggcc tcagcgccat ccgcgagcgc ctggggcccc tggtggaaca 3121 gggccgcgtg cgggccgcca ctgtgggctc cctggccggc cagccgctac aggagcgggc 3181 ccaggcctgg ggcgagcggc tgcgcgcgcg gatggaggag atgggcagcc ggacccgcga 3241 ccgcctggac gaggtgaagg agcaggtggc ggaggtgcgc gccaagctgg aggagcaggc 3301 ccagcagata cgcctgcagg ccgaggcctt ccaggcccgc ctcaagagct ggttcgagcc 3361 cctggtggaa gacatgcagc gccagtgggc cgggctggtg gagaaggtgc aggctgccgt 3421 gggcaccagc gccgcccctg tgcccagcga caatcactga acgccgaagc ctgcagccat 3481 gcgaccccac gccaccccgt gcctcctgcc tccgcgcagc ctgcagcggg agaccctgtc 3541 cccgccccag ccgtcctcct ggggtggacc ctagtttaat aaagattcac caagtttcac 3601 gca //