LOCUS       Exported                7861 bp DNA     linear   UNA 17-JUN-2022
DEFINITION  granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 7861)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2022 from SnapGene 5.3.2
            https://www.snapgene.com
COMMENT     gene ENSG00000030582
FEATURES             Location/Qualifiers
     source          1..7861
                     /organism="Homo sapiens"
                     /mol_type="genomic DNA"
     gene            1..7861
                     /label=GRN
                     /note="gene ENSG00000030582
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..4649
                     /label=GRN-212
                     /note="primary transcript ENST00000588170
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 17..4491
                     /label=GRN-215
                     /note="primary transcript ENST00000589536"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 20..5042
                     /label=GRN-209
                     /note="primary transcript ENST00000587518"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 20..4229
                     /label=GRN-207
                     /note="primary transcript ENST00000587109"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 37..5224
                     /label=GRN-211
                     /note="primary transcript ENST00000588143"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 53..5080
                     /label=GRN-221
                     /note="primary transcript ENST00000593167"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..7861
                     /label=GRN-201
                     /note="primary transcript ENST00000053867"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 57..5799
                     /label=GRN-213
                     /note="primary transcript ENST00000588237"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..5266
                     /label=GRN-208
                     /note="primary transcript ENST00000587387"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..5059
                     /label=GRN-220
                     /note="primary transcript ENST00000592783"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..5038
                     /label=GRN-218
                     /note="primary transcript ENST00000591740"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..4544
                     /label=GRN-219
                     /note="primary transcript ENST00000592323
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 57..4030
                     /label=GRN-203
                     /note="primary transcript ENST00000585512"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 60..7837
                     /label=GRN-214
                     /note="primary transcript ENST00000589265"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 61..4566
                     /label=GRN-210
                     /note="primary transcript ENST00000587958
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 69..5918
                     /label=GRN-206
                     /note="primary transcript ENST00000586782
                     Nonsense mediated decay"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5095,5197..5332,
                     5446..5555,5792..5918,6118..6215,6305..6550,6770..7003,
                     7096..7326,7416..7553)
                     /codon_start=1
                     /label=GRN-201
                     /note="coding sequence ENSP00000053867"
                     /note="This feature has 12 segments:
                         1: 3920 .. 4057 / #993366 / ENSE00003606043
                         2: 4181 .. 4306 / #993366 / ENSE00003646360
                         3: 4422 .. 4506 / #993366 / ENSE00002344685
                         4: 4983 .. 5095 / #993366 / ENSE00002337665
                         5: 5197 .. 5332 / #993366 / ENSE00003576251
                         6: 5446 .. 5555 / #993366 / ENSE00003466751
                         7: 5792 .. 5918 / #993366 / ENSE00003667214
                         8: 6118 .. 6215 / #993366 / ENSE00003647696
                         9: 6305 .. 6550 / #993366 / ENSE00003589157
                        10: 6770 .. 7003 / #993366 / ENSE00000732199
                        11: 7096 .. 7326 / #993366 / ENSE00000732197
                        12: 7416 .. 7553 / #993366 / ENSE00001376066"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVH
                     CCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCC
                     ELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD
                     VKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPH
                     QVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSD
                     HQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCP
                     SLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVK
                     DVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRRE
                     APRWDAPLRDPALRQLL"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5095,6305..6550,
                     6770..7003,7096..7326,7416..7553)
                     /codon_start=1
                     /label=GRN-214
                     /note="coding sequence ENSP00000467616"
                     /note="This feature has 8 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5095 / #993366 / ENSE00002337665
                        5: 6305 .. 6550 / #993366 / ENSE00003589157
                        6: 6770 .. 7003 / #993366 / ENSE00000732199
                        7: 7096 .. 7326 / #993366 / ENSE00000732197
                        8: 7416 .. 7553 / #993366 / ENSE00002870852"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIH
                     CCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCC
                     QLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSH
                     PRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEK
                     EVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRR
                     HCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQLL"
     prim_transcript 3920..7550
                     /label=GRN-222
                     /note="primary transcript ENST00000639447"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5095,5197..5332,
                     5446..5555,5792..5918,6118..6215,6305..6507,7284..7326,
                     7416..7550)
                     /codon_start=1
                     /label=GRN-222
                     /note="coding sequence ENSP00000492014"
                     /note="This feature has 11 segments:
                         1: 3920 .. 4057 / #993366 / ENSE00003804066
                         2: 4181 .. 4306 / #993366 / ENSE00003646360
                         3: 4422 .. 4506 / #993366 / ENSE00002344685
                         4: 4983 .. 5095 / #993366 / ENSE00002337665
                         5: 5197 .. 5332 / #993366 / ENSE00003576251
                         6: 5446 .. 5555 / #993366 / ENSE00003466751
                         7: 5792 .. 5918 / #993366 / ENSE00003667214
                         8: 6118 .. 6215 / #993366 / ENSE00003647696
                         9: 6305 .. 6507 / #993366 / ENSE00003806440
                        10: 7284 .. 7326 / #993366 / ENSE00003803882
                        11: 7416 .. 7550 / #993366 / ENSE00003807622"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVH
                     CCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCC
                     ELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGD
                     VKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPH
                     QVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCRDNRQGWACCPYRQGVCCAD
                     RRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQLL"
     CDS             join(3920..4057,4181..4306,5197..5332,5446..5555,
                     5792..5799)
                     /codon_start=1
                     /label=GRN-213
                     /note="coding sequence ENSP00000466611"
                     /note="This feature has 5 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 5197 .. 5332 / #993366 / ENSE00003576251
                        4: 5446 .. 5555 / #993366 / ENSE00003466751
                        5: 5792 .. 5799 / #993366 / ENSE00002805053"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEASCCEDRVHCCPHGAF
                     CDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKY
                     GCCPMPNAT"
     CDS             join(3920..4057,4181..4306,4380..4506,4983..5095,
                     5197..5266)
                     /codon_start=1
                     /label=GRN-208
                     /note="coding sequence ENSP00000467431"
                     /note="This feature has 5 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4380 .. 4506 / #993366 / ENSE00002856561
                        4: 4983 .. 5095 / #993366 / ENSE00002337665
                        5: 5197 .. 5266 / #993366 / ENSE00002950705"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEIKGPCQCRFLCVPQAV
                     ACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGC
                     CPMPQASCCEDRVHCCPHGAFCDLVHTR"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5095,
                     5197..5224)
                     /codon_start=1
                     /label=GRN-211
                     /note="coding sequence ENSP00000465375"
                     /note="This feature has 5 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00002891439
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5095 / #993366 / ENSE00002337665
                        5: 5197 .. 5224 / #993366 / ENSE00002926415"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVH
                     "
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5080)
                     /codon_start=1
                     /label=GRN-221
                     /note="coding sequence ENSP00000466405"
                     /note="This feature has 4 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5080 / #993366 / ENSE00002936527"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGC"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5059)
                     /codon_start=1
                     /label=GRN-220
                     /note="coding sequence ENSP00000467870"
                     /note="This feature has 4 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5059 / #993366 / ENSE00002824933"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVM"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5042)
                     /codon_start=1
                     /label=GRN-209
                     /note="coding sequence ENSP00000465518"
                     /note="This feature has 4 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5042 / #993366 / ENSE00002742660"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPDF"
     CDS             join(3920..4057,4181..4306,4422..4506,4983..5038)
                     /codon_start=1
                     /label=GRN-218
                     /note="coding sequence ENSP00000467022"
                     /note="This feature has 4 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4506 / #993366 / ENSE00002344685
                        4: 4983 .. 5038 / #993366 / ENSE00002882312"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRSCFQRSGNNSVGAIQCPDSQFECPD"
     CDS             join(3920..4057,4181..4306,4422..4491)
                     /codon_start=1
                     /label=GRN-215
                     /note="coding sequence ENSP00000466956"
                     /note="This feature has 3 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4306 / #993366 / ENSE00003646360
                        3: 4422 .. 4491 / #993366 / ENSE00002788927"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFH
                     CSADGRS"
     CDS             join(3920..4057,4181..4229)
                     /codon_start=1
                     /label=GRN-207
                     /note="coding sequence ENSP00000466271"
                     /note="This feature has 2 segments:
                        1: 3920 .. 4057 / #993366 / ENSE00003606043
                        2: 4181 .. 4229 / #993366 / ENSE00002835585"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGASYSCCRPL
                     LDKWPTTLSRHLGGPCQ"
     CDS             3920..4030
                     /codon_start=1
                     /label=GRN-203
                     /note="coding sequence ENSP00000467745"
                     /note="color: #993366"
                     /translation="MWTLVSWVALTAGLVAGTRCPDGQFCPVACCLDPGGA"
     primer_bind     4738..4757
                     /label=PCR Forward
                     /note="color: black; sequence: GAGCTGGGCCGGTCTAATAC; added:
                     2022-06-17"
     primer_bind     4738..4757
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: GAGCTGGGCCGGTCTAATAC; added:
                     2022-06-17"
     misc_feature    4942..5041
                     /label=Donor Template SNV -> REV
                     /note="color: #00ffff"
     primer_bind     4942..5041
                     /label=Donor Template SNV -> REV
                     /note="color: blue; sequence: 
                     cctgagtgggctggtagtatcctgggtcatcttgtccacagGTAACAACTCCGTGGGTG
                     CCATCCAGTGCCCTGATAGTCAGTTCGAATGCCCGGACTTC; added: 
                     2022-06-17"
     misc_feature    5001..5003
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    5004..5023
                     /label=Protospacer Sequence
                     /note="color: #ff0000"
     misc_feature    5006
                     /label=SNV
                     /note="WT = C
                     SNV = T"
                     /note="color: #ff9900"
     primer_bind     complement(5007..5023)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: CTGACTATCAGGGCACTAGA; added:
                     2022-06-17"
     prim_transcript 5044..6055
                     /label=GRN-217
                     /note="primary transcript ENST00000590984
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(5166..5185)
                     /label=PCR Reverese
                     /note="color: black; sequence: TGGTCCTGACTCCGTCTTCA; added:
                     2022-06-17"
     prim_transcript 5294..7798
                     /label=GRN-205
                     /note="primary transcript ENST00000586443"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(5294..5332,5446..5555,5792..5918,6118..6215,6305..6550,
                     6770..7003,7096..7407)
                     /codon_start=1
                     /label=GRN-205
                     /note="coding sequence ENSP00000465673"
                     /note="This feature has 7 segments:
                        1: 5294 .. 5332 / #993366 / ENSE00002819872
                        2: 5446 .. 5555 / #993366 / ENSE00003466751
                        3: 5792 .. 5918 / #993366 / ENSE00003667214
                        4: 6118 .. 6215 / #993366 / ENSE00003647696
                        5: 6305 .. 6550 / #993366 / ENSE00003589157
                        6: 6770 .. 7003 / #993366 / ENSE00000732199
                        7: 7096 .. 7407 / #993366 / ENSE00002970922"
                     /translation="WQRSSLPRGLTGQWPCPARSCVRTHGPGALMVLPAVSCPVGSMAA
                     AQCPTPPAAPITCTAAPKTLCVT*SRVSASPRRTLPRTSSLSCLRTQWGM*NVTWR*AA
                     QMAIPAAVYSRGPGAAALLPRLCAVRTTYTAVPRGLRVTRRRVPVNRGPTRCPGWRRPQ
                     LTSACQTHKP*REMSPVIMSAAVPPPIPAANSRLGSGAAVQSQRLSAARTTSTAAPRAT
                     RV*LRGSVSEEARSWLDWRRCLPAGLPYPTPETSAVTSTPAARWGRPAARAWVGAGPAA
                     SCPMLCAARIASTAARLATPAT*RLDPARRKWSLPSLPPSWPVALTWV*RTWSVGKDTS
                     AMITRPAAETTDRAGPAVPTARSVPTPILGLGMARDQVPPRPTLSPPS"
     prim_transcript 5527..6550
                     /label=GRN-216
                     /note="primary transcript ENST00000589923"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 5666..6354
                     /label=GRN-202
                     /note="primary transcript ENST00000585348
                     Retained intron"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 6957..7857
                     /label=GRN-204
                     /note="primary transcript ENST00000586242"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(6957..7003,7096..7326,7471..7524)
                     /codon_start=1
                     /label=GRN-204
                     /note="coding sequence ENSP00000467837"
                     /note="This feature has 3 segments:
                        1: 6957 .. 7003 / #993366 / ENSE00002866811
                        2: 7096 .. 7326 / #993366 / ENSE00000732197
                        3: 7471 .. 7524 / #993366 / ENSE00002867735"
                     /translation="AARAWVGAGPAASCPMLCAARIASTAARLATPAT*RLDPARRKWS
                     LPSLPPSWPVALTWV*RTWSVGKDTSAMITRPAAETTDRAGPAVPTASQGYQVFAQGGP
                     ALGRPF"
ORIGIN
        1 aaactgaggt aggcgggtca tcgcgctggg gtctgtagtc tgagcgctac ccggttgctg
       61 ctgcccaagg accgcggagt cggacgcagg taggagagcg gccgcgcaga cctctcgcct
      121 gctcctgccc aggggcccgc cagggccatg tgagcttgag gttcccctgg agtctcagcc
      181 ggagacaaca gaagaaccgc ttactgaaac tccttggggg ttctgataca ctagggggag
      241 ttttatggga aagaggaagc agtaattgca gtgacgcccc gttagaaggg gctttctacc
      301 tccccagcat tcccccaaag cagggaccac accattcttg acccagctcc acccctgtcg
      361 gtaggtgctg gcttcttccc ctctcctggt ggtggtgggt ggttcccgcg gcggcctgga
      421 gccggagggg cgcgcgaccc tgggctggga gctccgaggg cctgggaacg agacctgaga
      481 ccttggcttc tcgaaggtag tagggacttg ggagtggtga ctgaacctgg tctggctcct
      541 ccttacttcc tcttgttgcg ggtgggacga gctagcttcc gcctctccca gccacttttt
      601 cctgctcatt tgcagctagg ttggctcccc ttttgggaat ttcctctccc cttggcactc
      661 ggagttgggg ggtgccacct agtggaagat aacggagcta gggtcttgaa gaggctgctg
      721 tcccctctgg ctgttttggc ggtgtagggt ggcatgagag actgcgactc gcctcctcat
      781 ccctgtttct gtatgcgagt gcttgtattc agtagaagca tacactatac tccctcaatt
      841 tagggtaaac aggaggggcc acatgcacag gtaattcacc agggagccga acactcctgt
      901 gcagacagac tccccttccc agcaagccat ggcagcggac agcctgctga gaacacccag
      961 gaagcaggcg gtgccagctg caggtgcttt gcctgggagc tgtggggctg aggagagggt
     1021 ccactgtcca ggaccagtga acttcatcct tatctgtcca ggaggtggcc tcttggggat
     1081 gctgagttag gggaggggca cttgaggaaa gccaggtgga gcagagagga tgtgagtgac
     1141 tgggtgggtg agatttcctg cccctccccc cgcagtggta tccacaccta gactcgtggg
     1201 gtaactgagg cacagacaga gagcaacttc tcaggccctc acagttggca attctaggat
     1261 taggacccaa gtgcgatttt caggcagtcc ctgtaccctg tttctgttgt acctgttgca
     1321 ccattcccag gcactgccca tcgtgccact agtgatatga acccaggtcc aatacgctct
     1381 ggggccatca aagcctgacg tcaccatgac ctgatgtgtg acgtgttata ggtgtccctt
     1441 ggtatcttca cggaactggt tccaggaccc caaaatctgt gggtgctcaa gcccctgaga
     1501 taaaatggtg taatatttgc atataaccta tacatacttt aaatcatttc tagattactt
     1561 atacctaata caatggaaat gacatgtcgg ctgggcgtgg tggctcatgc ctgtaatccc
     1621 accactttgg gaggccgtgg caggtggatc acctgaggtc tggagtttga gaccagcctg
     1681 accaacatgg tgaaaccccc atctctacta aaaatacaaa aattagccag gtgtggtagc
     1741 gcacacctat aatcccacct acttgggagg ctgaggcagg agaattgctt gaacctggga
     1801 ggcggagttc gcagtaagct gagatcgcgc cactgtacta cagcctgggt gacagagcag
     1861 gactccatct caaaaaaaaa agagaaaaag aaaaagaaat gccatgtaaa tagttgtgat
     1921 cctgaattgt ttagggaata ataagaaaga actatctgta gatgttcagt atagatgcac
     1981 ccatcgtaag cctaactaca ttgtataact cagcaacgat gtaacatttt caggggtttt
     2041 tttgttttgt tttttgagac agaatctcag tctcactctg tcacccaggc tggagtatgt
     2101 tggcgtgatc tctgctcact gcaacctcca cctcctgggc tcaagcgatt ctcctgcctc
     2161 agcctcttga gtagctggga ttgcaggtgt gcgctaccac gcatggctaa tttttgtatt
     2221 tttaatagag atggggtttt accacgttgg tcaggctggt cttgaactcc tgaccttggg
     2281 atccgcccac ctgggcctcc caaagtgctg ggattacagg cgttagccac cgcgcccaat
     2341 atattttgat ccctggttgg atatggaggg ctgactgtac ttaacatctc taagcttcag
     2401 tttcctcctt taaaataaag gtgtggctgg gtgtggtggt tcaagcctgt aatcccagca
     2461 cttagggagg ctgaggtggg tggatcagct gaggtcagga gttcaagacc agcctgacca
     2521 atatggtgaa accccctctc tgctaaaaat acaaaaatta gccaggcgtg gtggcgagcg
     2581 cctgtagtcc cagctacttg cttgaacttg ggaggcagag gttgcagtga gctgagatcg
     2641 tgccactgaa ctcgagcatg ggcaacagag caagactgtc tcaaaaaaaa aaaaaaaaag
     2701 ggggtgagca gacgtggtgg cacgctccca cagtcccagc tacttagtag gaggccaagg
     2761 ttggaggatt gcttgatccc aggagtctga gtccagcctg ggcaacatgg caatacctca
     2821 tctctaaaaa taaaataaaa gtaaaggtat taattactac tttggatggt tgttgcaaag
     2881 aaatatatat aaaataatgg agagtcttgt aactggctcc caagaggctc aacagacatt
     2941 actgtttttg cttcttcatt atgagttacc tctctggcca ccccactgaa ctagctgggc
     3001 tagctgagcc tgggagaaga gttgtttagg aagtgagagg ctgctctcca cagagactca
     3061 aggctcagtt cctcctggtg actcagatgg gcagcccagt gggcacacgt ggtctctctc
     3121 cacatgtggc tgagtttcac ttccagaata gatggagagg caagggcagg gtttagcatg
     3181 cttgaggaat ctcagagggc cctggtggtg tgggggaccc tcagaacaca ggtgtctcaa
     3241 gggctgaccc agcttctgtg tccttttctc tgggtgagga ggggacattc atgggcagat
     3301 ggtgacctct ggggaaggca gcccagactc cactggccac catatttcct ttttcacaac
     3361 tttctcaccc ctgtggtttc ccatgtcatc atgtggccgc ttcccgcaag gccttagcgg
     3421 ggtgcaggta tgaacatagt gtcaggcaag gaggcatctg gaggggaacc ctggcttttc
     3481 ctggggggac tccctccctg caccctagcc ctgtcctctc ccatggctac tgatgccttc
     3541 ccctcacccc agaggtggcc cacatctgca cagatcagac ccacaaaaat cacgtcttcc
     3601 tgactctcat aagcctgccc agtgaggccc aggcattagg ccatgtgctg gggactcaga
     3661 cccacacata tacgcatgtc agcattcatg cttacaggtc cgcacatgct ggggcaagtg
     3721 tcacacacgg ggcgctgtag gaagctgact ctcagcccct gcagatttct gcctgcctgg
     3781 acagggaggt gttgagaagg ctcaggcagt cctgggccag gaccttggcc tggggctagg
     3841 gtactgagtg accctagaat caagggtggc gtgggcttaa gcagttgcca gacgttcctt
     3901 ggtactttgc aggcagacca tgtggaccct ggtgagctgg gtggccttaa cagcagggct
     3961 ggtggctgga acgcggtgcc cagatggtca gttctgccct gtggcctgct gcctggaccc
     4021 cggaggagcc agctacagct gctgccgtcc ccttctggtg agtgcccctc agcctaggca
     4081 agagctggca gcctgggttt tcccaaaggg tcatcttgga ttggccagag gaggacgcca
     4141 ggcacaagtc tgtggtttat cattttccct gtctttctag gacaaatggc ccacaacact
     4201 gagcaggcat ctgggtggcc cctgccaggt tgatgcccac tgctctgccg gccactcctg
     4261 catctttacc gtctcaggga cttccagttg ctgccccttc ccagaggtga gcgtgccatc
     4321 agcccagtgg aggggcttag gtctgcattt atgcttttcc tgcactctac cacctgcaga
     4381 taaaagggcc ctgccaatgc aggtttctct gtgttccaca ggccgtggca tgcggggatg
     4441 gccatcactg ctgcccacgg ggcttccact gcagtgcaga cgggcgatcc tgcttccaaa
     4501 gatcaggtgc agctggggtg tgggtgcagg gcaggcagac gggcagcatg tggagtctgg
     4561 aacccaggag cccagctggc gggggcagcc ctgattcctg cccttgtgcc ctcattcatg
     4621 tggcatctgt actaagcaac agccctgctg tggacagagg ggcagcactg gggataggag
     4681 ggtgcgggag aaagtgcaag actccaggtc caggcgttgt gggggtgggg agaggtcgag
     4741 ctgggccggt ctaataccaa cccatggtca gtgggtgccc cttccccatg ccatcttgct
     4801 gagggaggga ctggattgtg aggagggtga gttaggcctg cctaggagat cactgagcct
     4861 tagtgtcacc ctcaaacccc agtagctggg cttgcaggcc ctggtgccac cagctccttg
     4921 tgtgatgggg gagtcacctt ccctgagtgg gctggtagta tcctgggtca tcttgtccac
     4981 aggtaacaac tccgtgggtg ccatccagtg ccctgatagt cagttcgaat gcccggactt
     5041 ctccacgtgc tgtgttatgg tcgatggctc ctgggggtgc tgccccatgc cccaggtaca
     5101 aatctggggg agatgggggt atgtggaggg aagtgggggc agagttgggg gccaggggca
     5161 gggggtgaag acggagtcag gaccattttt tctcaggctt cctgctgtga agacagggtg
     5221 cactgctgtc cgcacggtgc cttctgcgac ctggttcaca cccgctgcat cacacccacg
     5281 ggcacccacc ccctggcaaa gaagctccct gcccagagga ctaacagggc aggtgaggag
     5341 gtgggagagc atcaggccag gggctggggc ggggcctcat tgactccaag tgtaggaaaa
     5401 agtttcctcc atcctggctg cccctcacgt ttgctcctct tccagtggcc ttgtccagct
     5461 cggtcatgtg tccggacgca cggtcccggt gccctgatgg ttctacctgc tgtgagctgc
     5521 ccagtgggaa gtatggctgc tgcccaatgc ccaacgtgag tgaggggctg gagccagctt
     5581 ggctgtgtgc ccccagccac ctggccctga cacgcacctt acaggggctc tgtggcatgg
     5641 ggctggctgg ctgcttgctg ggagcctggc tgatgcaggg ttcatgctac cccctagtgg
     5701 gggattgggg cagtgccagc catcagcctg gctgctccct gtgtgctact gagcctggaa
     5761 gtgacaaaga cccacccctg tccccactca ggccacctgc tgctccgatc acctgcactg
     5821 ctgcccccaa gacactgtgt gtgacctgat ccagagtaag tgcctctcca aggagaacgc
     5881 taccacggac ctcctcacta agctgcctgc gcacacaggt accagaggca gggtgcagat
     5941 acaggggtgg ggcccccttt cctccctttt aggcctggcc ttaggatcac tgcaaggtgg
     6001 tgtaagcggt accctccatc ttcaacacct ggttccagct gtggagccgg caaagggttg
     6061 atacccctga gggtccccag tgccacttct gacctgtcct ctctgcttcc ctcacagtgg
     6121 gggatgtgaa atgtgacatg gaggtgagct gcccagatgg ctatacctgc tgccgtctac
     6181 agtcgggggc ctggggctgc tgccctttta cccaggtacc caggggtggc gggtgggtgg
     6241 gctgagcaca gtgtggcagg cagccgggcc ccagtgccca cctgcccttc ttcatctgcc
     6301 ctaggctgtg tgctgtgagg accacataca ctgctgtccc gcggggttta cgtgtgacac
     6361 gcagaagggt acctgtgaac aggggcccca ccaggtgccc tggatggaga aggccccagc
     6421 tcacctcagc ctgccagacc cacaagcctt gaagagagat gtcccctgtg ataatgtcag
     6481 cagctgtccc tcctccgata cctgctgcca actcacgtct ggggagtggg gctgctgtcc
     6541 aatcccagag gtatatggga ggggacagca tcttggcctg ggcaggtggg tggccaagct
     6601 cctattgctt tctgccctcc gcatagccca taggtgatac ccagctctga cagattcgtc
     6661 cccagctgga ggtgctgtaa gcaggagagg cgggctggag taggtagggg ctcggcactg
     6721 cgccccacat agtggctacc tacaacgccc tttcctgccc accccccagg ctgtctgctg
     6781 ctcggaccac cagcactgct gcccccaggg ctacacgtgt gtagctgagg ggcagtgtca
     6841 gcgaggaagc gagatcgtgg ctggactgga gaagatgcct gcccgccggg cttccttatc
     6901 ccaccccaga gacatcggct gtgaccagca caccagctgc ccggtggggc agacctgctg
     6961 cccgagcctg ggtgggagct gggcctgctg ccagttgccc catgtgagtg cctccctgcc
     7021 tgcccctgga taggggagct aagcccagtg aggggacagg aacataatgc cattctgtgc
     7081 tcccttcccc gccaggctgt gtgctgcgag gatcgccagc actgctgccc ggctggctac
     7141 acctgcaacg tgaaggctcg atcctgcgag aaggaagtgg tctctgccca gcctgccacc
     7201 ttcctggccc gtagccctca cgtgggtgtg aaggacgtgg agtgtgggga aggacacttc
     7261 tgccatgata accagacctg ctgccgagac aaccgacagg gctgggcctg ctgtccctac
     7321 cgccaggtca gtgccaaccc ccatcctggg gctgggtatg gccagggacc aggtcccacc
     7381 tcgtccaacc ctctcgcccc cctctgacca tccagggcgt ctgttgtgct gatcggcgcc
     7441 actgctgtcc tgctggcttc cgctgcgcag ccaggggtac caagtgtttg cgcagggagg
     7501 ccccgcgctg ggacgcccct ttgagggacc cagccttgag acagctgctg tgagggacag
     7561 tactgaagac tctgcagccc tcgggacccc actcggaggg tgccctctgc tcaggcctcc
     7621 ctagcacctc cccctaacca aattctccct ggaccccatt ctgagctccc catcaccatg
     7681 ggaggtgggg cctcaatcta aggccttccc tgtcagaagg gggttgtggc aaaagccaca
     7741 ttacaagctg ccatcccctc cccgtttcag tggaccctgt ggccaggtgc ttttccctat
     7801 ccacaggggt gtttgtgtgt gtgcgcgtgt gcgtttcaat aaagtttgta cactttctta
     7861 a
//
