LOCUS Exported 3410 bp DNA linear UNA 14-JUN-2022 DEFINITION profilin 1 [Source:HGNC Symbol;Acc:HGNC:8881]. ACCESSION . VERSION . KEYWORDS . SOURCE Homo sapiens ORGANISM Homo sapiens REFERENCE 1 (bases 1 to 3410) AUTHORS Ensembl TITLE Direct Submission JOURNAL Exported Jun 14, 2022 from SnapGene 5.3.2 https://www.snapgene.com COMMENT gene ENSG00000108518 FEATURES Location/Qualifiers source 1..3410 /organism="Homo sapiens" /mol_type="genomic DNA" gene 1..3410 /label=PFN1 /note="gene ENSG00000108518 Protein coding" /note="color: #a6acb3; direction: RIGHT" prim_transcript 1..2369 /label=PFN1-202 /note="primary transcript ENST00000572383" /note="color: #a6acb3; direction: RIGHT" CDS join(43..118,507..799,2242..2369) /codon_start=1 /label=PFN1-202 /note="coding sequence ENSP00000460363" /note="This feature has 3 segments: 1: 43 .. 118 / #993366 / ENSE00002633594 2: 507 .. 799 / #993366 / ENSE00002668429 3: 2242 .. 2369 / #993366 / ENSE00002678014" /translation="MHSRPRRRRRRGHTRRRPPVLPFGGGSGGARAAAQRAEPRSGRQR VPRALRLPPPASRGSSSPVRGPSSSAESSPSSSAMAGWNAYIDNLMADGTCQDAAIVGY KDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEF SM" prim_transcript 532..3410 /label=PFN1-201 /note="primary transcript ENST00000225655" /note="color: #ff9900; direction: RIGHT" CDS join(668..799,2242..2434,3065..3162) /codon_start=1 /label=PFN1-201 /note="coding sequence ENSP00000225655" /note="This feature has 3 segments: 1: 668 .. 799 / #993366 / ENSE00001324890 2: 2242 .. 2434 / #993366 / ENSE00000676461 3: 3065 .. 3162 / #993366 / ENSE00001488299" /translation="MAGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITP AEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVT KTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY" prim_transcript 1593..3395 /label=PFN1-203 /note="primary transcript ENST00000574872" /note="color: #a6acb3; direction: RIGHT" primer_bind 2193..2217 /label=PCR Forward /note="color: black; sequence: GAATCTTGGTGCACTGACTAACTTG; added: 2022-06-15" primer_bind 2209..2228 /label=Sanger Sequencing Primer /note="color: black; sequence: ACTAACTTGATGGGCGCTTG; added: 2022-06-15" CDS join(2218..2434,3065..3162) /codon_start=1 /label=PFN1-203 /note="coding sequence ENSP00000465019" /note="This feature has 2 segments: 1: 2218 .. 2434 / #993366 / ENSE00002681343 2: 3065 .. 3162 / #993366 / ENSE00002644033" /translation="MGAWFLFQPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQD GEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY " misc_feature 2284..2383 /label=Donor Template WT -> SNV /note="color: #00ffff" primer_bind complement(2284..2383) /label=Donor Template WT -> SNV /note="color: blue; sequence: TCTTGGTACGAAGATCCATGCTAAATTCCCCATCCTGCAGCAGTGAGTCCCGGATCACC GAACCTTTCTGGCCCCCAAGTGTCAGCCCATTCACGTAAAA; added: 2022-06-15" misc_feature 2313..2315 /label=PAM /note="color: #ffff00" misc_feature 2316..2335 /label=Protospacer Sequence /note="color: #ff0000" primer_bind complement(2316..2335) /label=gRNA Protospacer /note="color: black; sequence: CCCGGATCACCGAACATTTC; added: 2022-06-15" misc_feature 2320 /label=SNV /note="WT=T SNV=G" /note="color: #ff9900" primer_bind complement(2728..2752) /label=PCR Reverse /note="color: black; sequence: AAGAACTCAAACGATGAACTCGATG; added: 2022-06-15" prim_transcript complement(join(2852..3410,1..970)) /label=ENO3-204 /note="primary transcript ENST00000519266 Protein coding" /note="color: #a6acb3; direction: LEFT" prim_transcript complement(join(6158..3410,1..810)) /label=ENO3-209 /note="primary transcript ENST00000520221 Protein coding" /note="color: #a6acb3; direction: LEFT" gene complement(join(8069..3410,1..970)) /label=ENO3 /note="gene ENSG00000108515 Protein coding" /note="color: #a6acb3; direction: LEFT" ORIGIN 1 cgggcgcgct cccgtgcagc cggctccggc cccgaccgcc ccatgcactc ccggccccgg 61 cgcaggcgca ggcgcgggca cacgcgccgc cgcccgccgg tccttccctt cggcggaggt 121 gggggaagga ggagtcatcc cgtttaaccc tgggctcccc gaactctcct taatttgcta 181 aatttgcagc ttgctaattc ctcctgcttt ctccttcctt ccttcttctg gctcactccc 241 tgccccgata ccaaagtctg gtttatattc agtgcaaatt ggagcaaacc ctacccttca 301 cctctctccc gccacccccc atccttctgc attgctttcc atcgaactct gcaaattttg 361 caataggggg agggattttt aaaattgcat ttgcaaagtt cggtgtctgg gctggcgagt 421 gggggaggga gggaatgggg agtaggcccc gcccctaccg tcctttgcaa ataaaaatct 481 agcggggcgg ggggggggag gagcaggaag tggcggtgcg agggctgctg cacagcgagc 541 ggagccgcgg tccggacggc agcgcgtgcc ccgagctctc cgcctccccc cgcccgccag 601 ccgaggcagc tcgagcccag tccgcggccc cagcagcagc gccgagagca gccccagtag 661 cagcgccatg gccgggtgga acgcctacat cgacaacctc atggcggacg ggacctgtca 721 ggacgcggcc atcgtgggct acaaggactc gccctccgtc tgggccgccg tccccgggaa 781 aacgttcgtc aacatcacgg tactgcgagg cctgcgcggt ccggggcact gctccggttt 841 ggaccctgag ggagggactt gggtgggggc gggcgcggtc tagggcgcgc agcgagaggc 901 cctgactggg tgcttgccct ggaagcggcg cgaatggcgg ctgcacgtga gtgggtccct 961 cagttgcccg taggtcctat gccccaggcg gcggcgccca tcccgcccgc taccccgctt 1021 tccgcgacgg ggcgttacat ccggggcaca gggcggggag gggcgcggcg cccggtgcgg 1081 cggcccactt ccgcctctcc cagggcgggg cggggacgcg cctcagtttt tactggggat 1141 tgggattgag aggggacccg gggatccttg gacccccagc tccccgtccg gctgccagcc 1201 cgcgtccctc taccattgtt tctcctggtg cggaagggag ccccaggcgc gtggtcttgg 1261 tcccttgccc accctctgtg ggcttctggc ccctcctaac tccgttagag agggcgaggc 1321 caccacacgc ccccccagct cagcacgttg gattcaacat tcctgcgagg ccaatcccgg 1381 aagtcccctc gccctccctt cccgccatcc ggcggggcct ggagcagggg aactttgtga 1441 gaagttctga gacagcataa gcgaaagtat attggtttac taccgctgct cccaccacca 1501 tctccctgtc cacactgccc ggaagtgggg agggggagcg gaagttcaaa agggggggga 1561 agggggtttc cgggcgggag gggaccggat gtgggggctt ggggtgaaag ggggaggggg 1621 gaaaggttcc cggggtacct agacccgaga gctggcctgc cgggtctggc ccctgtccac 1681 cttcaaaagt tttccaactt tcccggaaag cccccagggg tctgcttcct catctcagag 1741 cagagatacc tggagctaaa ctctaggtta cttggagacc caggcgtccg ggccctaaca 1801 gctcctgcag aacctttgac caaataaggg caaagttgct tctcttgcct cgcccgcccc 1861 cctcccttcc cctctccgct tctgcttttc ctgaaggaga gttctgagca tccaaaggct 1921 cctcacttac ctcactgact ccctttattt cctgggttta ctttccccat taacactgac 1981 atctaaatct ctagaggagt atgtcatccc ttcctccctg gttccatatt ctaagtttca 2041 gaatcatgat ctttttctgc actaacgtcc cacagtcctc agagtttctc acagttcatt 2101 gatgtgtact gagtatataa tttatactta gcccccacgg cggggtggtt ctcagaagtg 2161 gaagacaggt gggtctttgg agaacacggt gggaatcttg gtgcactgac taacttgatg 2221 ggcgcttggt tcctcttcca gccagctgag gtgggtgtcc tggttggcaa agaccggtca 2281 agtttttacg tgaatgggct gacacttggg ggccagaaat gttcggtgat ccgggactca 2341 ctgctgcagg atggggaatt tagcatggat cttcgtacca agagcaccgg tggggccccc 2401 accttcaatg tcactgtcac caagactgac aagagtgagt tcctgagtct tacccttcct 2461 ttagctccaa gatctctaat gtaccgcctt ctggtaatct tgagggtgct tttttgtgtg 2521 attccccaac atgaagggag tcagaccagt attgtgttcc ttgtctgctg gatgctggaa 2581 cacgggatag tccctccatt ctgtctcagt tcctcatctt gtaattcgaa gttgctgaac 2641 tgcattacct cttaaattac ttactgcctt gagctcggat tagaaactgt tttctaggag 2701 tataggtaga ttgttggggt cttgaatcat cgagttcatc gtttgagttc ttttgttaga 2761 acctggaggt ggcatgcaca tccacagccc tttctagcct gaacacatga atcaacagtc 2821 tatgagcctt tgacccctaa gtttttagtc tgttcctctc cagcgtttaa gcttgatttt 2881 cctcttttga aaagccttat tgtttcaggg tgactgacag cctgcctgtg tgtggtgggg 2941 gggcgggttg ggagagatga ggttgggtta cagcccccag ggctggacag ctgctgaaca 3001 gctggcaggg ggtggaattg tgacacctgg atcctagcct cctttctctt tcctcctcct 3061 ccagcgctag tcctgctgat gggcaaagaa ggtgtccacg gtggtttgat caacaagaaa 3121 tgttatgaaa tggcctccca ccttcggcgt tcccagtact gacctcgtct gtcccttccc 3181 cttcaccgct ccccacagct ttgcacccct ttcctcccca tacacacaca aaccatttta 3241 ttttttgggc cattacccca taccccttat tgctgccaaa accacatggg ctgggggcca 3301 gggctggatg gacagacacc tccccctacc catatccctc ccgtgtgtgg ttggaaaact 3361 tttgtttttt ggggtttttt ttttctgaat aaaaaagatt ctactaacaa //