Recoil Wobbler 3 Jackson, a remutation on proximal Chromosome 10
Son Yong Karst, Patricia F. Ward-Bailey, Leah Rae Donahue, Kenneth R. Johnson, and Muriel T. Davisson
Source of Support: This research was supported by NIH/NCRR grant RR01183 to the Mouse Mutant Resource (M.T.Davisson, PI) and Cancer Center Core Grant CA34196.
Mutation (allele) symbol: rcw3J
Mutation (allele) name: recoil wobbler 3 Jackson
Gene symbol: Grm1rcw-3J
Strain of origin: C57BL/6J
Current strain name: C57BL/6J-rcw3J/J
Stock #: 005521 (view JAX® Mice Data Sheet for additional information including Price and Supply Information) Note: As of 1-4-07 available cryopreserved from JAX® Mice or as DNA only from the Jackson Laboratory DNA Resource.
Phenotype categories: Neurological: motor capabilities/coordination/movement
Abstract
We have identified a new remutation to recoil wobbler (rcw) (MGD 2005)by a direct test for allelism. The new neurological spontaneous mutation named recoil wobbler 3 Jackson causes the same phenotype, described below, as the original rcw mutation and the rcw2J allele. (MMR web site 2004)
Origin and Description
The recoil wobbler 3 Jackson mutation was discovered by Daniel Myrick in a production colony of C57BL/6J at the Jackson Laboratory in 2004. This spontaneous mutation has recessive inheritance and homozygous mutants are recognized at 2 weeks of age by their slightly smaller body size and wobbly gait. Like rcw/rcw mice, the rcw3J/rcw3J mutants often loose their balance when walking and constantly wobble back and forth. The original rcw/rcw mutants also have the slightly smaller body size. Homozygous mutant females breed moderately, but males are very poor breeders. The colony is maintained by homozygous mutant x heterozygote, or by heterozygote x heterozygote matings. Mutant mice live normal life spans.
Genetic Analysis
This mutation has recessive inheritance as shown by mating a C57BL/6J-rcw3J/J homozygous female mouse to an unrelated +/+ male of the CAST/Ei strain. The F1 progeny from this mating were unaffected, but affected animals were observed in the F2 progeny.
A direct test for allelism was set up by mating C57BL/6J-rcw3J females heterozygous for this new mutation with B6.129X1Baxtm1sjk/J-rcw2J/J heterozygous males. This mating produced 3 affected progeny out of 11 born, confirming that the two mutations are allelic.
Pathology
In a routine pathological screening of two homozygous mutants at 12 weeks of age, no gross lesions were observed.
Hearing as assessed by Auditory Brainstem Response testing (ABR) testing in two homozygous, one heterozygous and one control littermate at 10 weeks of age was normal.
The eyes of two homozygous mutants were examined with an opthalamascope and were found to be normal. An electroretinogram (ERG) test of two homozygous mutants was also normal.
Discussion
A direct test for allelism confirmed that this mutation is allelic with recoil wobbler (rcw). This remutation is available from JAXMice and the JAX DNA Resource. Sperm are being cryopreserved.
Acknowledgements
The authors thank Heping Yu for hearing assessment, Norm Hawes for the eye examination and Ronald Hurd for the ERG test. We also thank Jane Maynard for excellent technical skills.
References
MGD
Mouse Genome Database (MGD) Mouse Genome Informatics Project, The Jackson Laboratory, Bar Harbor, Maine. World Wide Web
http://www.informatics.jax.org
MMR website
