Beige 16 Jackson: a remutation of the Lyst gene

Richard M. Samples, Patricia F. Ward-Bailey, Leah Rae Donahue, Roderick T. Bronson, and Muriel T. Davisson

Source of Support: The research was supported by NIH/NCRR grant RR01183 to the Mouse Mutant Resources (M.T.Davisson, PI) and Cancer Center Core Grant CA34196.

Mutation (allele) symbol: Lystbg-16J


Mutation (allele) name: beige 16 Jackson

Gene symbol: Lyst

Strain of origin: B6.129S7-Rag1tm1Mom/J

Current strain name: B6(129S7) Rag1tm1Mom-Lystbg-16J/J

Stock #: 006126 (Available as DNA only from the Jackson Laboratory DNA Resource)

Phenotype categories: coat color

Abstract

A spontaneous, recessive remutation of Lystbghas been discovered and named beige 16 Jackson (Lystbg-16J).The mutation causes affected mice to have a lighter coat color than non-affected littermates. A direct test for allelism confirmed that this new mutation is an allele of Lystbg.

Origin and Description

Mice carrying this new remutation to Lystbg were discovered by Sabrina Gifford in a production colony of B6.129S7-Rag1tm1Mom/J mice (stock # 002216) at the Jackson Laboratory on June 08, 2005. Mice homozygous for this spontaneous, recessive mutation are recognized by a diluted coat color. (See Photo) Like the original Lystbg mutation this new remutation also lightens the color of the eyes, feet, ears, and tail in affected animals.

Genetic Analysis

A direct test for allelism was accomplished by mating a homozygous B6(129S7) Rag1tm1Mom-Lystbg-16J/J male mouse to two SB/LeJ (stock# 000269) females (Lystbg / Lystbg )homozygous for satin and beige. These matings produced 36 offspring in 5 litters and all 36 animals displayed the beige phenotype (See Photo) proving that this new remutation is an allele of Lystbg.

Pathology

Hearing as assessed by auditory brainstem response (ABR) testing of one homozygous mutant at two months of age revealed no hearing loss.

The eyes of 1 homozygous mutant mouse at two months of age were tested with an opthalmascope and showed pigment loss in the iris and retina which is typical of mice carrying the beige mutation.

Acknowledgements

The authors wish to thank Sabrina Gifford for the discovery of the mutant, Heping Yu for the hearing test, Norm Hawes for the eye examination, and Coleen Marden for her excellent technical assistance.