Uoxtm1Bay Allopurinol Therapy

Therapy necessary for homozygous breeding units and their wean or segregating litters from heterozygous breeder pairs.

  1. Obtain allopurinol from Sigma Chemicals #A8003 25 gm bottle - $60.00.
  2. Send a sample of each chemical lot to Diagnostic for microbe assay (routine procedure at The Jackson Laboratory)
  3. Measure out 150 mg allopurinol. Please following warning label on bottle: may sensitize skin; should not be inhaled or swallowed.
  4. Fill to 1 liter (1000ml) with water from bottles. Final concentration 0.15 mg/ml.
  5. Close container and shake to mix. Refill bottles for mice. To conserve useage fill bottles - 3/4 full. Water should be yellowish so bottles containing the mixture can be identified from regular water bottles. if there is a bottle run-out over the weekend, a regular bottle can be used as replacement--note this for the attending caretakers--and put back on allupurinol as soon as possible the following week.
  6. Store unused allopurinol solution in the refrigerator. Make up only as needed because 1) light breaks down the chemical and 2) chemical loses activity over time.


Note: There is a range of effective concentrations. Please refer to the following reference for more information.

Wu X, Wakamiya M, Vaishnav S, Geske R, Montgomery C, Jones P, Bradley A, Caskey CT. 1994. Hyperuricemia and urate nephropathy in urate oxidase-deficient mice. Proc Natl Acad Sci USA 91:742-746.