Colony maintenance for Ts65Dn strains

The Ts65Dn strains (001924 and 005252) are maintained by crossing Ts65Dn trisomic females to B6EiC3SnF1/J (001875) or (C57BL/6JEiJ x C3Sn.BLiA-Pde6b+/DnJ)F1/J (003647) males, respectively. Ts65Dn females generally breed well with an average litter size of 5-8 and the mothers take good care of their pups. The environment seems to have a profound effect on these mice. They are on a fourteen hour light and 10 hour dark cycle, mated as single pairs, with mates kept together throughout their six month breeding rotation, and noise is kept to a minimum. It is preferable to have them maintained and handled by one person. The diet is especially important, as malnourished mothers will not nurse their pups. Most facilities provide maintenance and breeding chows and Ts65Dn should be on the latter. Ts65Dn mice at The Jackson Laboratory are fed the LabDiet® 5K52/5K67 diet with a 6% fat content.

The incidence of trisomy varies among litters (ranging from 20-50%) with some females transmitting the trisomy more readily than others. Ts65Dn males are functionally sterile although the numbers of sperm vary from male to male. The recessive retinal degeneration 1 mutation (Pdeb rd1) is segregating in the 001924 colony which results in blindness in homozygotes, whereas the 005252 colony is homozygous wild-type for Pde6b.

All progeny are genotyped by PCR or qPCR, see Protocols for details.

This Resource is supported by NICHD/NIH contract #HHSN275201000006.