Colony Maintenance Ts65Dn females X B6EiC3Sn (C57BL/6JEi x C3H/HeSnJ) F1 males
The Ts65Dn stock is maintained by crossing Ts65Dn females to C57BL/6JEi x C3H/HeSnJ (B6EiC3Sn) F1 males. Ts65Dn females generally breed well with an average litter size of 5-8 and the mothers take good care of their pups. The environment seems to have a profound effect on these mice. They are on a fourteen hour light and 10 hour dark cycle, mated as single pair matings (male is not removed when female is pregnant) and noise is kept to a minimum. It is preferable to have them maintained and handled by one person. The diet is especially important, as malnourished mothers will not nurse their pups. Most facilities provide maintenance and breeding chows and Ts65Dn should be on the latter. The animals at TJL are fed NIH31 diet with a 6% fat content. The incidence of trisomy is about 20% although some females seem to transmit the trisomy more readily than others. Ts65Dn males are functionally sterile although the numbers of sperm vary from male to male. The recessive retinal degeneration 1 mutation (Pdeb rd1) is segregating in this colony. All progeny are genotyped by qPCR.
This Resource is supported by NICHD/NIH contract #N01-HD-73265.
